Click image to see more details
Product Info Summary
| SKU: | A01974-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD7 Antibody Picoband®
SKU/Catalog Number
A01974-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD7 Antibody Picoband® catalog # A01974-2. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01974-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD7 recombinant protein (Position: A26-D172).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01974-2 is reactive to CD7 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30-40 kDa
Calculated molecular weight
30570 MW
Background of CD7
CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01974-2 is guaranteed for ELISA, Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
IHC: human tonsil tissue
FCM: PBMC cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of CD7 using anti-CD7 antibody (A01974-2).
CD7 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD7 Antibody (A01974-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. Flow Cytometry analysis of PBMC cells using anti-CD7 antibody (A01974-2).
Overlay histogram showing PBMC cells stained with A01974-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD7 Antibody (A01974-2,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 3. Western blot analysis of CD7 using anti-CD7 antibody (A01974-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CD7 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD7 antigen affinity purified polyclonal antibody (Catalog # A01974-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD7 at approximately 30-40KD. The expected band size for CD7 is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For CD7 (Source: Uniprot.org, NCBI)
Gene Name
CD7
Full Name
T-cell antigen CD7
Weight
30570 MW
Alternative Names
T-cell antigen CD7; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41; CD7; CD7 CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell CD7|CD7 (p41)|T-cell leukemia |T-cell surface Leu-9|p41 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD7, check out the CD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD7 Antibody Picoband® (A01974-2)
Hello CJ!
No publications found for A01974-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD7 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD7 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-CD7 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CD7 antibody A01974-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
We purchased anti-CD7 antibody for Flow Cytometry on leukemic t-cell in the past. I am using human, and We want to use the antibody for ICC next. My question regards examining leukemic t-cell as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
I have checked the website and datasheets of our anti-CD7 antibody and it appears that A01974-2 has been tested on human in both Flow Cytometry and ICC. Thus A01974-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-18
Question
Is this A01974-2 anti-CD7 antibody reactive to the isotypes of CD7?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The immunogen of A01974-2 anti-CD7 antibody is E. coli-derived human CD7 recombinant protein (Position: A26-D172). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-25
Question
I see that the anti-CD7 antibody A01974-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-20
Question
Is there a BSA free version of anti-CD7 antibody A01974-2 available?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD7 antibody A01974-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-CD7 antibody A01974-2 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-CD7 antibody (A01974-2) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
Question
I was wanting to use your anti-CD7 antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
As indicated on the product datasheet, A01974-2 anti-CD7 antibody has been tested for ELISA, Flow Cytometry, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-28
Question
Does anti-CD7 antibody A01974-2 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to the expression profile of leukemic t-cell, CD7 is highly expressed in leukemic t-cell. So, it is likely that anti-CD7 antibody A01974-2 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-05-23
Question
I have a question about product A01974-2, anti-CD7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Li
Verified customer
Asked: 2019-05-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01974-2 anti-CD7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-08
Question
We have observed staining in human muscle. Any tips? Is anti-CD7 antibody supposed to stain muscle positively?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
According to literature muscle does express CD7. According to Uniprot.org, CD7 is expressed in blood, muscle, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD7 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-03
Question
We need to test anti-CD7 antibody A01974-2 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD7 antibody A01974-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Krishna
Verified customer
Asked: 2018-07-10
Answer
The products we sell, including anti-CD7 antibody A01974-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD7 antibody A01974-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-30
Question
Is a blocking peptide available for product anti-CD7 antibody (A01974-2)?
J. Wu
Verified customer
Asked: 2017-05-08
Answer
We do provide the blocking peptide for product anti-CD7 antibody (A01974-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-08
Question
My colleagues were content with the WB result of your anti-CD7 antibody. However we have seen positive staining in leukemic t-cell membrane using this antibody. Is that expected? Could you tell me where is CD7 supposed to be expressed?
T. Li
Verified customer
Asked: 2015-06-22
Answer
From what I have seen in literature, leukemic t-cell does express CD7. Generally CD7 expresses in membrane. Regarding which tissues have CD7 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-06-22
Question
Our lab want to know about using your anti-CD7 antibody for transmembrane receptor protein tyrosine kinase signaling pathway studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
W. Gonzalez
Verified customer
Asked: 2013-03-13
Answer
I appreciate your inquiry. This A01974-2 anti-CD7 antibody is validated on human tonsil tissue, pbmc cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-03-13
Question
Does A01974-2 anti-CD7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Li
Verified customer
Asked: 2013-03-13
Answer
It shows on the product datasheet, A01974-2 anti-CD7 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-13
