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Product Info Summary
| SKU: | PB9951 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ISG15 Antibody Picoband®
View all ISG15/UCRP Antibodies
SKU/Catalog Number
PB9951
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ISG15 Antibody Picoband® catalog # PB9951. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ISG15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9951)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse ISG15 recombinant protein (Position: A2-G155). Mouse ISG15 shares 65.8% amino acid (aa) sequence identity with human ISG15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9951 is reactive to Isg15 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
17898 MW
Background of ISG15/UCRP
Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9951 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse spleen tissue
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISG15/Ucrp antigen affinity purified polyclonal antibody (Catalog # PB9951) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ISG15/Ucrp at approximately 17 kDa. The expected band size for ISG15/Ucrp is at 18 kDa.
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Figure 2. IHC analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951).
ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ISG15/Ucrp Antibody (PB9951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Isg15 (Source: Uniprot.org, NCBI)
Gene Name
Isg15
Full Name
Ubiquitin-like protein ISG15
Weight
17898 MW
Alternative Names
Ubiquitin-like protein ISG15;Interferon-induced 15 kDa protein;Interferon-induced 17 kDa protein;IP17;Ubiquitin cross-reactive protein;Isg15;G1p2, Ucrp; ISG15 G1P2, IFI15, IMD38, IP17, UCRP, hUCRP ISG15 ubiquitin like modifier ubiquitin-like protein ISG15|interferon, alpha-inducible protein (clone IFI-15K)|interferon-induced 17-kDa/15-kDa protein|interferon-stimulated protein, 15 kDa|ubiquitin cross-reactive protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Isg15, check out the Isg15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Isg15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ISG15 Antibody Picoband® (PB9951)
Hello CJ!
PB9951 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
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Customer Q&As
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4 Customer Q&As for Anti-ISG15 Antibody Picoband®
Question
Will anti-ISG15 antibody PB9951 work on canine IHC with liver?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Our lab technicians have not validated anti-ISG15 antibody PB9951 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ISG15 antibody PB9951 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
Do you have a BSA free version of anti-ISG15 antibody PB9951 available?
J. Parker
Verified customer
Asked: 2019-11-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ISG15 antibody PB9951 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-15
Question
Will PB9951 anti-ISG15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
You can see on the product datasheet, PB9951 anti-ISG15 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-ISG15 antibody PB9951 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on canine tissues as well?
M. Johnson
Verified customer
Asked: 2013-01-10
Answer
The anti-ISG15 antibody (PB9951) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-10
