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Product Info Summary
| SKU: | A07595 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EFNA3/Ephrin A3 Antibody
SKU/Catalog Number
A07595
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EFNA3/Ephrin A3 Antibody (Catalog # A07595). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EFNA3/Ephrin A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07595)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human EFNA3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07595 is reactive to EFNA3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
26350 MW
Background of Ephrin-A3
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling By similarity.
Kozlosky C.J., Oncogene 10:299-306(1995).
Davis S., Science 266:816-819(1994).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07595 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and Jurkat cells, using EFNA3 antibody A07595.
Protein Target Info & Infographic
Gene/Protein Information For EFNA3 (Source: Uniprot.org, NCBI)
Gene Name
EFNA3
Full Name
Ephrin-A3
Weight
26350 MW
Superfamily
ephrin family
Alternative Names
Ephrin-A3;EFL-2;EHK1 ligand;EHK1-L;EPH-related receptor tyrosine kinase ligand 3;LERK-3;EFNA3;EFL2, EPLG3, LERK3; EFNA3 EFL2, EPLG3, Ehk1-L, LERK3 ephrin A3 ephrin-A3|EFL-2|EHK1 ligand|LERK-3|eph-related receptor tyrosine kinase ligand 3|ligand of eph-related kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFNA3, check out the EFNA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFNA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EFNA3/Ephrin A3 Antibody (A07595)
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No publications found for A07595
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Customer Q&As
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5 Customer Q&As for Anti-EFNA3/Ephrin A3 Antibody
Question
We are currently using anti-EFNA3/Ephrin A3 antibody A07595 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
The anti-EFNA3/Ephrin A3 antibody (A07595) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-15
Question
I see that the anti-EFNA3/Ephrin A3 antibody A07595 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-08
Question
I have a question about product A07595, anti-EFNA3/Ephrin A3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07595 anti-EFNA3/Ephrin A3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
I am looking for to test anti-EFNA3/Ephrin A3 antibody A07595 on human skin of abdomen for research purposes, then I may be interested in using anti-EFNA3/Ephrin A3 antibody A07595 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Krishna
Verified customer
Asked: 2015-01-06
Answer
The products we sell, including anti-EFNA3/Ephrin A3 antibody A07595, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-01-06
Question
Does A07595 anti-EFNA3/Ephrin A3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Wu
Verified customer
Asked: 2013-08-09
Answer
You can see on the product datasheet, A07595 anti-EFNA3/Ephrin A3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-09
