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Product Info Summary
| SKU: | M01822 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRF6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01822
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF6 Rabbit Monoclonal Antibody catalog # M01822. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01822)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GHD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01822 is reactive to IRF6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
53130 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01822 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of IRF6 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IRF6 (Source: Uniprot.org, NCBI)
Gene Name
IRF6
Full Name
Interferon regulatory factor 6
Weight
53130 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 6;IRF-6;IRF6; IRF6 LPS, OFC6, PIT, PPS, PPS1, VWS, VWS1 interferon regulatory factor 6 interferon regulatory factor 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF6, check out the IRF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF6 Rabbit Monoclonal Antibody (M01822)
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No publications found for M01822
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-IRF6 Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for placenta using anti-IRF6 Rabbit Monoclonal antibody M01822. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-06
Question
Would anti-IRF6 Rabbit Monoclonal antibody M01822 work on canine IF with secondary oocyte?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
Our lab technicians have not tested anti-IRF6 Rabbit Monoclonal antibody M01822 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IRF6 Rabbit Monoclonal antibody M01822 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine secondary oocyte in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-06
Question
We are currently using anti-IRF6 Rabbit Monoclonal antibody M01822 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
The anti-IRF6 Rabbit Monoclonal antibody (M01822) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-15
Question
I have a question about product M01822, anti-IRF6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01822 anti-IRF6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-28
Question
Would M01822 anti-IRF6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Roberts
Verified customer
Asked: 2016-07-25
Answer
As indicated on the product datasheet, M01822 anti-IRF6 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-25
Question
I see that the anti-IRF6 Rabbit Monoclonal antibody M01822 works with WB, what is the protocol used to produce the result images on the product page?
S. Williams
Verified customer
Asked: 2013-07-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-07-02
