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Product Info Summary
| SKU: | M00958-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IRF5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00958-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF5 Rabbit Monoclonal Antibody catalog # M00958-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00958-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GGD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00958-1 is reactive to IRF5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
56044 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00958-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of IRF5 expression in Ramos cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IRF5 (Source: Uniprot.org, NCBI)
Gene Name
IRF5
Full Name
Interferon regulatory factor 5
Weight
56044 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 5;IRF-5;IRF5; IRF5 SLEB10 interferon regulatory factor 5 interferon regulatory factor 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF5, check out the IRF5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF5 Rabbit Monoclonal Antibody (M00958-1)
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No publications found for M00958-1
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Customer Q&As
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5 Customer Q&As for Anti-IRF5 Rabbit Monoclonal Antibody
Question
I see that the anti-IRF5 Rabbit Monoclonal antibody M00958-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-26
Question
Will anti-IRF5 Rabbit Monoclonal antibody M00958-1 work on bovine WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
Our lab technicians have not tested anti-IRF5 Rabbit Monoclonal antibody M00958-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IRF5 Rabbit Monoclonal antibody M00958-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cortex of kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-26
Question
Do you have a BSA free version of anti-IRF5 Rabbit Monoclonal antibody M00958-1 available?
T. Jones
Verified customer
Asked: 2017-07-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF5 Rabbit Monoclonal antibody M00958-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-18
Question
See attached the WB image, lot number and protocol we used for cortex of kidney using anti-IRF5 Rabbit Monoclonal antibody M00958-1. Please let me know if you require anything else.
J. Moore
Verified customer
Asked: 2015-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-24
Question
Would anti-IRF5 Rabbit Monoclonal antibody M00958-1 work for WB with cortex of kidney?
F. Parker
Verified customer
Asked: 2013-05-02
Answer
According to the expression profile of cortex of kidney, IRF5 is highly expressed in cortex of kidney. So, it is likely that anti-IRF5 Rabbit Monoclonal antibody M00958-1 will work for WB with cortex of kidney.
Boster Scientific Support
Answered: 2013-05-02
