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Product Info Summary
| SKU: | M00207 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Rho A Rabbit Monoclonal Antibody
SKU/Catalog Number
M00207
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rho A Rabbit Monoclonal Antibody catalog # M00207. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rho A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FAC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rho A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00207 is reactive to RHOA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated molecular weight
21768 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00207 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human U251 whole cell, human SIHA whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rho A using anti-Rho A antibody (M00207).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rho A antigen affinity purified monoclonal antibody (Catalog # M00207) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rho A at approximately 22 kDa. The expected band size for Rho A is at 22 kDa.
Click image to see more details
Immunofluorescent analysis of Jurkat cells, using Rho A Antibody.
Protein Target Info & Infographic
Gene/Protein Information For RHOA (Source: Uniprot.org, NCBI)
Gene Name
RHOA
Full Name
Transforming protein RhoA
Weight
21768 MW
Superfamily
small GTPase superfamily
Alternative Names
Transforming protein RhoA;Rho cDNA clone 12;h12;RHOA;ARH12, ARHA, RHO12; RHOA ARH12, ARHA, EDFAOB, RHO12, RHOH12 ras homolog family member A transforming protein RhoA|Aplysia ras-related homolog 12|epididymis secretory sperm binding protein|oncogene RHO H12|small GTP binding protein RhoA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOA, check out the RHOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rho A Rabbit Monoclonal Antibody (M00207)
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7 Customer Q&As for Anti-Rho A Rabbit Monoclonal Antibody
Question
Is this M00207 anti-Rho A Rabbit Monoclonal antibody reactive to the isotypes of RHOA?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The immunogen of M00207 anti-Rho A Rabbit Monoclonal antibody is A synthesized peptide derived from human Rho A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-18
Question
Here is the WB image, lot number and protocol we used for metanephric glomerulus using anti-Rho A Rabbit Monoclonal antibody M00207. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-Rho A Rabbit Monoclonal antibody (M00207)?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
We do provide the blocking peptide for product anti-Rho A Rabbit Monoclonal antibody (M00207). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-22
Question
I was wanting to use your anti-Rho A Rabbit Monoclonal antibody for IF for human metanephric glomerulus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human metanephric glomerulus identification?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
As indicated on the product datasheet, M00207 anti-Rho A Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human metanephric glomerulus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-21
Question
Do you have a BSA free version of anti-Rho A Rabbit Monoclonal antibody M00207 available?
J. Carter
Verified customer
Asked: 2016-09-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Rho A Rabbit Monoclonal antibody M00207 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-09-26
Question
Will anti-Rho A Rabbit Monoclonal antibody M00207 work on dog IF with brain?
R. Evans
Verified customer
Asked: 2016-07-12
Answer
Our lab technicians have not tested anti-Rho A Rabbit Monoclonal antibody M00207 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Rho A Rabbit Monoclonal antibody M00207 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-12
Question
My question regarding product M00207, anti-Rho A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Anderson
Verified customer
Asked: 2016-02-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00207 anti-Rho A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-04
