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Product Info Summary
| SKU: | A00580 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IRF-1 Antibody
SKU/Catalog Number
A00580
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IRF-1 Antibody catalog # A00580. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00580)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human IRF1. AA range:91-140
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00580 is reactive to IRF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
36502 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00580 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of PC12 cells using IRF-1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For IRF1 (Source: Uniprot.org, NCBI)
Gene Name
IRF1
Full Name
Interferon regulatory factor 1
Weight
36502 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 1;IRF-1;IRF1; IRF1 IRF-1, MAR interferon regulatory factor 1 interferon regulatory factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF1, check out the IRF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF-1 Antibody (A00580)
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No publications found for A00580
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IRF-1 Antibody
Question
We have been able to see staining in mouse blood. Any tips? Is anti-IRF-1 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
From literature blood does express IRF1. From Uniprot.org, IRF1 is expressed in blood, placenta, pancreas, among other tissues. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447
Boster Scientific Support
Answered: 2019-08-27
Question
We are currently using anti-IRF-1 antibody A00580 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The anti-IRF-1 antibody (A00580) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-26
Question
We need to test anti-IRF-1 antibody A00580 on rat blood for research purposes, then I may be interested in using anti-IRF-1 antibody A00580 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The products we sell, including anti-IRF-1 antibody A00580, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-IRF-1 antibody A00580 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
According to the expression profile of blood, IRF1 is highly expressed in blood. So, it is likely that anti-IRF-1 antibody A00580 will work for WB with blood.
Boster Scientific Support
Answered: 2019-08-06
Question
Is this A00580 anti-IRF-1 antibody reactive to the isotypes of IRF1?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
The immunogen of A00580 anti-IRF-1 antibody is Synthesized peptide derived from the Internal region of human IRF-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-21
Question
Does A00580 anti-IRF-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
You can see on the product datasheet, A00580 anti-IRF-1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-19
Question
Our team were content with the WB result of your anti-IRF-1 antibody. However we have been able to see positive staining in pancreas nucleus. cytoplasm. note=myd88-associated using this antibody. Is that expected? Could you tell me where is IRF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
From what I have seen in literature, pancreas does express IRF1. Generally IRF1 expresses in nucleus. cytoplasm. note=myd88-associated. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447
Boster Scientific Support
Answered: 2018-07-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IRF-1 antibody A00580. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-14
Question
I was wanting to use your anti-IRF-1 antibody for WB for rat blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat blood identification?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
As indicated on the product datasheet, A00580 anti-IRF-1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-30
Question
I see that the anti-IRF-1 antibody A00580 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-11
Question
Is there a BSA free version of anti-IRF-1 antibody A00580 available?
D. Baker
Verified customer
Asked: 2017-08-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IRF-1 antibody A00580 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-03
Question
Here is the WB image, lot number and protocol we used for blood using anti-IRF-1 antibody A00580. Please let me know if you require anything else.
P. Jackson
Verified customer
Asked: 2017-05-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-08
Question
Is a blocking peptide available for product anti-IRF-1 antibody (A00580)?
M. Jackson
Verified customer
Asked: 2014-09-02
Answer
We do provide the blocking peptide for product anti-IRF-1 antibody (A00580). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-09-02
Question
My lab would like using your anti-IRF-1 antibody for dna-templated studies. Has this antibody been tested with western blotting on pc12 cells? We would like to see some validation images before ordering.
S. Moore
Verified customer
Asked: 2013-12-17
Answer
Thanks for your inquiry. This A00580 anti-IRF-1 antibody is tested on pc12 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-12-17
Question
My question regarding product A00580, anti-IRF-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Wu
Verified customer
Asked: 2013-10-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00580 anti-IRF-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-10-01
