Product Info Summary
SKU: | M01247 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRAK4 Monoclonal Antibody
SKU/Catalog Number
M01247
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRAK4 Monoclonal Antibody catalog # M01247. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01247)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABIH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRAK4 Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01247 is reactive to IRAK4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
59144 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01247 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IRAK4 expression in Jurkat cell lysate.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For IRAK4 (Source: Uniprot.org, NCBI)
Gene Name
IRAK4
Full Name
Interleukin-1 receptor-associated kinase 4
Weight
59144 MW
Superfamily
protein kinase superfamily
Alternative Names
Occludin;OCLN; IRAK4 IMD67, IPD1, IRAK-4, NY-REN-64, REN64 interleukin 1 receptor associated kinase 4 interleukin-1 receptor-associated kinase 4|renal carcinoma NY-REN-64
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRAK4, check out the IRAK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRAK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRAK4 Monoclonal Antibody (M01247)
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No publications found for M01247
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Customer Q&As
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1 Customer Q&As for Anti-IRAK4 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01247?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-IRAK4 Monoclonal Antibody (M01247) expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2019-12-10