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Product Info Summary
| SKU: | M04787 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IQGAP2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04787
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IQGAP2 Rabbit Monoclonal Antibody catalog # M04787. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IQGAP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04787)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
29I69
Isotype
IgG
Immunogen
A synthesized peptide derived from human IQGAP2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04787 is reactive to IQGAP2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
181 kDa
Calculated molecular weight
181 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04787 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, mouse stomach tissue, mouse brain tissue
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IQGAP2 using anti-IQGAP2 antibody (M04787).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: mouse stomach tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP2 antigen affinity purified monoclonal antibody (Catalog # M04787) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP2 at approximately 181 kDa. The expected band size for IQGAP2 is at 181 kDa.
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Figure 2. IHC analysis of IQGAP2 using anti-IQGAP2 antibody (M04787).
IQGAP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IQGAP2 Antibody (M04787) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IQGAP2 (Source: Uniprot.org, NCBI)
Gene Name
IQGAP2
Full Name
Ras GTPase-activating-like protein IQGAP2
Weight
181 kDa
Alternative Names
Alpha-sarcoglycan;Alpha-SG;50 kDa dystrophin-associated glycoprotein;50DAG;Adhalin;Dystroglycan-2;SGCA;ADL, DAG2; IQGAP2 IQ motif containing GTPase activating protein 2 ras GTPase-activating-like protein IQGAP2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IQGAP2, check out the IQGAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IQGAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IQGAP2 Rabbit Monoclonal Antibody (M04787)
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