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Product Info Summary
| SKU: | M00772-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00772-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody catalog # M00772-2. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00772-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFI-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin beta 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00772-2 is reactive to ITGB1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated molecular weight
88415 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00772-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human bladder carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human bladder carcinoma, using Integrin beta 1 Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of using Integrin beta1 expression in U937 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ITGB1 (Source: Uniprot.org, NCBI)
Gene Name
ITGB1
Full Name
Integrin beta-1
Weight
88415 MW
Superfamily
integrin beta chain family
Alternative Names
Integrin beta-1;Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29;ITGB1;FNRB, MDF2, MSK12; ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB integrin subunit beta 1 integrin beta-1|glycoprotein IIa|integrin VLA-4 beta subunit|integrin beta 1|integrin, beta 1 (fibronectin receptor, beta polypeptide, CD29 includes MDF2, MSK12)|very late activation protein, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGB1, check out the ITGB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody (M00772-2)
Hello CJ!
M00772-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody
Question
Will anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 work for IHC with skin?
C. Johnson
Verified customer
Asked: 2019-11-21
Answer
According to the expression profile of skin, ITGB1 is highly expressed in skin. So, it is likely that anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 will work for IHC with skin.
Boster Scientific Support
Answered: 2019-11-21
Question
I see that the anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-22
Question
My lab would like to test anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 on human skin for research purposes, then I may be interested in using anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The products we sell, including anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2. Let me know if you need anything else.
A. Mitchell
Verified customer
Asked: 2019-04-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-25
Question
Do you have a BSA free version of anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 available?
L. Mangal
Verified customer
Asked: 2018-11-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-13
Question
Will anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 work on zebrafish IHC with skeletal muscle?
D. Wu
Verified customer
Asked: 2018-08-31
Answer
Our lab technicians have not validated anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish skeletal muscle in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-31
Question
I was wanting to use your anti-Integrin beta 1 Rabbit Monoclonal antibody for IHC for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Z. Wu
Verified customer
Asked: 2015-06-16
Answer
As indicated on the product datasheet, M00772-2 anti-Integrin beta 1 Rabbit Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-16
