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Product Info Summary
| SKU: | A01933-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Integrin alpha 2/ITGA2 Antibody Picoband®
View all Integrin alpha 2/CD49b Antibodies
SKU/Catalog Number
A01933-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Integrin alpha 2/ITGA2 Antibody Picoband® catalog # A01933-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Integrin alpha 2/ITGA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01933-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Integrin alpha 2/ITGA2 recombinant protein (Position: E285-S1181).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01933-2 is reactive to ITGA2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
165 kDa
Calculated molecular weight
129.295kDa
Background of Integrin alpha 2/CD49b
Integrin alpha-2, or CD49b (cluster of differentiation 49b), is a protein which in humans is encoded by the CD49b gene. This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01933-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A549 whole cell
FCM: A375 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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Figure 2. Flow Cytometry analysis of A375 cells using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2).
Overlay histogram showing A375 cells stained with A01933-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Alpha 2/ITGA2 Antibody (A01933-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ITGA2 (Source: Uniprot.org, NCBI)
Gene Name
ITGA2
Full Name
Integrin alpha-2
Weight
129.295kDa
Superfamily
integrin alpha chain family
Alternative Names
Mitochondrial fission 1 protein; FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11; FIS1; TTC11; CGI-135 ITGA2 BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2 integrin subunit alpha 2 integrin alpha-2|CD49 -like family member B|alpha 2 subunit of VLA-2 receptor|collagen receptor|human platelet allo system 5|integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)|platelet Br|platelet glycoprotein GPIa|platelet membrane glycoprotein Ia|very late activation protein 2 receptor, alpha-2 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGA2, check out the ITGA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin alpha 2/ITGA2 Antibody Picoband® (A01933-2)
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