Anti-Importin subunit alpha-3 KPNA4 Antibody

Product Name

Anti-Importin subunit alpha-3 KPNA4 Antibody

View all Importin alpha 3/KPNA4 Antibodies

SKU/Catalog Number

A05188

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Importin subunit alpha-3 KPNA4 Antibody (Catalog # A05188). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KPNA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Importin subunit alpha-3 KPNA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05188)

Host

Rabbit

Contents

KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4. The immunogen is located within amino acids 30 - 80 of KPNA4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Recognizes SIRP alpha 1, 2 and 3.

Reactive Species

A05188 is reactive to KPNA4 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

57887 MW

Background of Importin alpha 3/KPNA4

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α-induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05188 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For KPNA4 (Source: Uniprot.org, NCBI)

Alternative Names

QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1 KPNA4 IPOA3, QIP1, SRP3 karyopherin subunit alpha 4 importin subunit alpha-3|importin alpha Q1|importin subunit alpha-4|karyopherin alpha 4 (importin alpha 3)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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