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Product Info Summary
| SKU: | PB9571 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband®
SKU/Catalog Number
PB9571
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® catalog # PB9571. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9571)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CCL3 recombinant protein (Position: S24-A92). Human CCL3 shares 75.8% and 77.3% amino acid (aa) sequence identity with mouse and rat CCL3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9571 is reactive to CCL3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
10085 MW
Background of CCL3
Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for CCL3 predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. CCL3 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. CCL3 is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9571 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CCL3 (Source: Uniprot.org, NCBI)
Gene Name
CCL3
Full Name
C-C motif chemokine 3
Weight
10085 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 3;G0/G1 switch regulatory protein 19-1;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;PAT 464.1;SIS-beta;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein;MIP-1-alpha (4-69);LD78-alpha (4-69);CCL3;G0S19-1, MIP1A, SCYA3; CCL3 G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 C-C motif chemokine ligand 3 C-C motif chemokine 3|G0/G1 switch regulatory protein 19-1|PAT 464.1|SIS-beta|macrophage inflammatory protein 1-alpha|small inducible cytokine A3 (homologous to mouse Mip-1a)|tonsillar lymphocyte LD78 alpha protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL3, check out the CCL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® (PB9571)
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No publications found for PB9571
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Customer Q&As
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3 Customer Q&As for Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband®
Question
I see that the anti-CCL3/Macrophage Inflammatory Protein 1 alpha antibody PB9571 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-CCL3/Macrophage Inflammatory Protein 1 alpha antibody PB9571 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The anti-CCL3/Macrophage Inflammatory Protein 1 alpha antibody (PB9571) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-26
Question
I was wanting to use your anti-CCL3/Macrophage Inflammatory Protein 1 alpha antibody for ELISA for human substantia nigra on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human substantia nigra identification?
B. Walker
Verified customer
Asked: 2019-11-14
Answer
You can see on the product datasheet, PB9571 anti-CCL3/Macrophage Inflammatory Protein 1 alpha antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human substantia nigra in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
