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Product Info Summary
| SKU: | M00021 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-IL10 Monoclonal Antibody
SKU/Catalog Number
M00021
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IL10 Monoclonal Antibody catalog # M00021. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL10 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00021)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOIG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL10 Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII, leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00021 is reactive to IL10 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00021 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human U251 whole cell, human Jurkat whole cell, rat spleen tissue, rat PC-12 whole cell, mouse spleen tissue, mouse RAW264.7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (M00021).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified monoclonal antibody (M00021) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa. The expected band size for IL10 is at 21 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
88068 MW
Superfamily
IL-10 family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL10 Monoclonal Antibody (M00021)
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Customer Q&As
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15 Customer Q&As for Anti-IL10 Monoclonal Antibody
Question
My team were content with the WB result of your anti-IL10 Monoclonal antibody. However we have observed positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
According to literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-IL10 Monoclonal antibody (M00021)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do provide the blocking peptide for product anti-IL10 Monoclonal antibody (M00021). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-10
Question
My question regarding product M00021, anti-IL10 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00021 anti-IL10 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
We have seen staining in human esophagus. Do you have any suggestions? Is anti-IL10 Monoclonal antibody supposed to stain esophagus positively?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to literature esophagus does express IL10. According to Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-12-24
Question
I was wanting to use your anti-IL10 Monoclonal antibody for IF for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It shows on the product datasheet, M00021 anti-IL10 Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
Is there a BSA free version of anti-IL10 Monoclonal antibody M00021 available?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 Monoclonal antibody M00021 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-05
Question
We ordered your anti-IL10 Monoclonal antibody for IF on t-cell in a previous experiment. I am using human, and We want to use the antibody for WB next. I am looking for examining t-cell as well as esophagus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
C. Collins
Verified customer
Asked: 2019-10-30
Answer
I took a look at the website and datasheets of our anti-IL10 Monoclonal antibody and I see that M00021 has been validated on human in both IF and WB. Thus M00021 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-30
Question
Will M00021 anti-IL10 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Thomas
Verified customer
Asked: 2019-04-12
Answer
As indicated on the product datasheet, M00021 anti-IL10 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-12
Question
Is this M00021 anti-IL10 Monoclonal antibody reactive to the isotypes of IL10?
F. Anderson
Verified customer
Asked: 2019-03-04
Answer
The immunogen of M00021 anti-IL10 Monoclonal antibody is A synthesized peptide derived from human IL10 Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII, leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-04
Question
Will anti-IL10 Monoclonal antibody M00021 work for IF with t-cell?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
According to the expression profile of t-cell, IL10 is highly expressed in t-cell. So, it is likely that anti-IL10 Monoclonal antibody M00021 will work for IF with t-cell.
Boster Scientific Support
Answered: 2019-01-29
Question
I see that the anti-IL10 Monoclonal antibody M00021 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-05
Question
We are currently using anti-IL10 Monoclonal antibody M00021 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
H. Walker
Verified customer
Asked: 2017-12-05
Answer
The anti-IL10 Monoclonal antibody (M00021) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-05
Question
See below the WB image, lot number and protocol we used for t-cell using anti-IL10 Monoclonal antibody M00021. Please let me know if you require anything else.
L. Miller
Verified customer
Asked: 2017-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-21
Question
I was wanting to use to test anti-IL10 Monoclonal antibody M00021 on human t-cell for research purposes, then I may be interested in using anti-IL10 Monoclonal antibody M00021 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Krishna
Verified customer
Asked: 2013-10-18
Answer
The products we sell, including anti-IL10 Monoclonal antibody M00021, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL10 Monoclonal antibody M00021. Let me know if you need anything else.
B. Li
Verified customer
Asked: 2013-02-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-19
