Anti-Interleukin-4 IL4 Antibody Picoband®

IL-4 antibody

Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # PA1529. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 4 publication(s).

Product Info Summary

SKU: PA1529
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Interleukin-4 IL4 Antibody Picoband®

View all IL-4 Antibodies

SKU/Catalog Number

PA1529

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # PA1529. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-4 IL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1529)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1529 is reactive to Il4 in Mouse

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

15834 MW

Background of IL-4

Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo, in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1529 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Il4 (Source: Uniprot.org, NCBI)

Gene Name

Il4

Full Name

Interleukin-4

Weight

15834 MW

Superfamily

IL-4/IL-13 family

Alternative Names

Interleukin-4;IL-4;B-cell IgG differentiation factor;B-cell growth factor 1;B-cell stimulatory factor 1;BSF-1;IGG1 induction factor;Lymphocyte stimulatory factor 1;Il4;Il-4; IL4 BCGF-1, BCGF1, BSF-1, BSF1, IL-4 interleukin 4 interleukin-4|B cell growth factor 1|B_cell stimulatory factor 1|binetrakin|interleukin 4 variant 2|lymphocyte stimulatory factor 1|pitrakinra

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Il4, check out the Il4 Infographic

Il4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Il4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1529 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Tan Y, Liu H, Yang H, Wang L, Qin X. J Physiol Sci. 2013 Jan;63(1):63-9. Doi: 10.1007/S12576-012-0231-X. Epub 2012 Sep 26. An Inactivated Pseudomonas Aeruginosa Medicament Inhibits Airway Allergic Inflammation And Improves Epithelial Functions.

Wang Yz, Feng Xg, Shi Qg, Hao Yl, Yang Y, Zhang Am, Kong Qx. Inflammation. 2013 Apr;36(2):337-45. Doi: 10.1007/S10753-012-9551-5. Silencing Of Mir155 Promotes The Production Of Inflammatory Mediators In Guillain-Barr?? Syndrome In Vitro.

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

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3 Customer Q&As for Anti-Interleukin-4 IL4 Antibody Picoband®

Question

We have been able to see staining in mouse oocyte. Do you have any suggestions? Is anti-IL4 antibody supposed to stain oocyte positively?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

From what I have seen in literature oocyte does express IL4. From what I have seen in Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-17

Question

Our lab were content with the WB result of your anti-IL4 antibody. However we have seen positive staining in oocyte secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?

M. Parker

Verified customer

Asked: 2016-08-04

Answer

Based on literature, oocyte does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-08-04

Question

We are currently using anti-IL4 antibody PA1529 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on primate tissues as well?

T. Kulkarni

Verified customer

Asked: 2013-10-04

Answer

The anti-IL4 antibody (PA1529) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-10-04

Order DetailsPrice
PA1529

100μg

$370
PA1529-10ug

10μg sample (liquid)

$99
PA1529-Biotin

100 μg Biotin conjugated

$570
PA1529-Cy3

100 μg Cy3 conjugated

$570
PA1529-Dylight488

100 μg Dylight488 conjugated

$570
PA1529-Dylight550

100 μg Dylight550 conjugated

$570
PA1529-Dylight594

100 μg Dylight594 conjugated

$570
PA1529-FITC

100 μg FITC conjugated

$570
PA1529-HRP

100 μg HRP conjugated

$570
PA1529-APC

100 μg APC conjugated

$670
PA1529-PE

100 μg PE conjugated

$670
PA1529-iFluor647

100 μg iFluor647 conjugated

$670
PA1529-carrier-free

Carrier Free

$370

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