Product Info Summary
SKU: | A01918S176-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-IK alpha/beta (S176/177) CHUK Antibody
SKU/Catalog Number
A01918S176-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IK alpha/beta (S176/177) CHUK Antibody catalog # A01918S176-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IK alpha/beta (S176/177) CHUK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01918S176-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177. AA range:151-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01918S176-2 is reactive to CHUK in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
84640 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01918S176-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of NIH-3T3 cells using Phospho-IKKα/β (S176/177) polyclonal antibody diluted at 1:1000
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The picture was kindly provided by our customer, antibody was diluted at 1:500
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Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CHUK (Source: Uniprot.org, NCBI)
Gene Name
CHUK
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Weight
84640 MW
Superfamily
protein kinase superfamily
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;2.7.11.10;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B inhibitor kinase alpha;NFKBIKA;Transcription factor 16;TCF-16;CHUK;IKKA, TCF16; CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHUK, check out the CHUK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHUK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IK alpha/beta (S176/177) CHUK Antibody (A01918S176-2)
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