Anti-IKB beta/NFKBIB Antibody Picoband®

Product Name

Anti-IKB beta/NFKBIB Antibody Picoband®

View all IkB-beta Antibodies

SKU/Catalog Number

PB9292

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IKB beta/NFKBIB Antibody Picoband® catalog # PB9292. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IKB beta/NFKBIB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9292)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9292 is reactive to NFKBIB in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

37771 MW

Background of IkB-beta

NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9292 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Mouse Kidney Tissue, RH35 Whole Cell, NRK Whole Cell, HELA Whole Cell
IHC: Human Intestinal Cancer Tissue, Mouse Intestine Tissue, Rat Intestine Tissue

Validation Images & Assay Conditions

Gene/Protein Information For NFKBIB (Source: Uniprot.org, NCBI)

Alternative Names

NF-kappa-B inhibitor beta;NF-kappa-BIB;I-kappa-B-beta;IkB-B;IkB-beta;IkappaBbeta;Thyroid receptor-interacting protein 9;TR-interacting protein 9;TRIP-9;NFKBIB;IKBB, TRIP9; NFKBIB IKBB, TRIP9 NFKB inhibitor beta NF-kappa-B inhibitor beta|I-kappa-B-beta|NF-kappa-BIB|TR-interacting protein 9|TRIP-9|ikB-B|ikB-beta|ikappaBbeta|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta|thyroid receptor-interacting protein 9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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