Anti-Ikaros/IKZF1 Antibody Picoband®

Ikaros/IKZF1 antibody

Boster Bio Anti-Ikaros/IKZF1 Antibody Picoband® catalog # PB9643. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9643
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Ikaros/IKZF1 Antibody Picoband®

View all Ikaros/IKZF1 Antibodies

SKU/Catalog Number

PB9643

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ikaros/IKZF1 Antibody Picoband® catalog # PB9643. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ikaros/IKZF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9643)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9643 is reactive to IKZF1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50-70 kDa

Calculated molecular weight

57528 MW

Background of Ikaros/IKZF1

DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9643 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human Daudi whole cell
IHC: mouse spleen tissue, rat spleen tissue, human tonsil tissue
ICC/IF: MCF-7 cell
FCM: U937 cell

Validation Images & Assay Conditions

Gene/Protein Information For IKZF1 (Source: Uniprot.org, NCBI)

Gene Name

IKZF1

Full Name

DNA-binding protein Ikaros

Weight

57528 MW

Superfamily

Ikaros C2H2-type zinc-finger protein family

Alternative Names

DNA-binding protein Ikaros;Ikaros family zinc finger protein 1;Lymphoid transcription factor LyF-1;IKZF1;IK1, IKAROS, LYF1, ZNFN1A1; IKZF1 CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1 IKAROS family zinc finger 1 DNA-binding protein Ikaros|CLL-associated KW-6|ikaros family zinc finger protein 1|lymphoid transcription factor LyF-1|protein phosphatase 1, regulatory subunit 92|zinc finger protein, subfamily 1A, 1 (Ikaros)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IKZF1, check out the IKZF1 Infographic

IKZF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IKZF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Ikaros/IKZF1 Antibody Picoband®

Question

We are currently using anti-Ikaros/IKZF1 antibody PB9643 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

R. Anderson

Verified customer

Asked: 2020-04-30

Answer

The anti-Ikaros/IKZF1 antibody (PB9643) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-30

Question

I see that the anti-Ikaros/IKZF1 antibody PB9643 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-18

Question

I was wanting to use your anti-Ikaros/IKZF1 antibody for IHC for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

It shows on the product datasheet, PB9643 anti-Ikaros/IKZF1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-02

Question

Does anti-Ikaros/IKZF1 antibody PB9643 work on bovine IHC with thymus?

T. Rodriguez

Verified customer

Asked: 2014-10-28

Answer

Our lab technicians have not tested anti-Ikaros/IKZF1 antibody PB9643 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Ikaros/IKZF1 antibody PB9643 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine thymus in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-28

Order DetailsPrice
PB9643

100μg

$370
PB9643-10ug

10μg sample (liquid)

$99
PB9643-Biotin

100 μg Biotin conjugated

$570
PB9643-Cy3

100 μg Cy3 conjugated

$570
PB9643-Dylight488

100 μg Dylight488 conjugated

$570
PB9643-Dylight550

100 μg Dylight550 conjugated

$570
PB9643-Dylight594

100 μg Dylight594 conjugated

$570
PB9643-FITC

100 μg FITC conjugated

$570
PB9643-HRP

100 μg HRP conjugated

$570
PB9643-APC

100 μg APC conjugated

$670
PB9643-PE

100 μg PE conjugated

$670
PB9643-iFluor647

100 μg iFluor647 conjugated

$670
PB9643-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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