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Product Info Summary
| SKU: | PA1498 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IGFBP3 Antibody Picoband®
SKU/Catalog Number
PA1498
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGFBP3 Antibody catalog # PA1498. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1498)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IGFBP-3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1498 is reactive to IGFBP3 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
31674 MW
Background of IGFBP-3
IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1498 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: 293T Whole Cell, MCF-7 Whole Cell, A549 Whole Cell, SW620 Whole Cell, rat liver tissue, rat kidney tissue, rat heart tissue
Validation Images & Assay Conditions
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Anti-IGFBP-3 antibody, PA1498, Western blotting
All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 40KD
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Figure 2. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (PA1498).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # PA1498) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40KD. The expected band size for IGFBP3 is at 32KD.
Protein Target Info & Infographic
Gene/Protein Information For IGFBP3 (Source: Uniprot.org, NCBI)
Gene Name
IGFBP3
Full Name
Insulin-like growth factor-binding protein 3
Weight
31674 MW
Alternative Names
Insulin-like growth factor-binding protein 3;IBP-3;IGF-binding protein 3;IGFBP-3;IGFBP3;IBP3; IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGFBP3, check out the IGFBP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGFBP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGFBP3 Antibody Picoband® (PA1498)
Hello CJ!
PA1498 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Correlation between insulin%u2011like growth factor binding protein 3 and metastasis%u2011associated gene 1 protein in esophageal squamous cell carcinoma
Pu Xy, Zheng Xg, Zhang Y, Xiao Hj, Xu Zp, Liu Jm, Wang Hp, Wen Am, Zhou Xx, Wu Yl. J Sex Med. 2011 Aug;8(8):2181-90. Doi: 10.1111/J.1743-6109.2011.02318.X. Epub 2011 May 19. Higher Expression Of Mrna And Protein Of Insulin-Like Growth Factor Bindi...
Pu Xy, Wen Am, Liu Jm, Zheng Xg, Xiao Hj, Xu Zp, Wang Hp, Li D, Zhang Rl. J Urol. 2014 Sep;192(3):990-6. Doi: 10.1016/J.Juro.2014.02.042. Epub 2014 Feb 24. Shrna Constructs Targeting Igfbp-3 Alleviate Age Related Erectile Dysfunction In The Rat.
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Customer Q&As
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1 Customer Q&As for Anti-IGFBP3 Antibody Picoband®
Question
Can PA1498 detect mouse IGFBP3?
Verified customer
Asked: 2019-04-25
Answer
The WB and IHC test result on mouse samples are not good. We do not recommend using the Anti-IGFBP3 Antibody (PA1498) on mouse samples.
Boster Scientific Support
Answered: 2019-05-08
