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Product Info Summary
| SKU: | A02633-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IFITM1 Antibody Picoband®
SKU/Catalog Number
A02633-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IFITM1 Antibody Picoband® catalog # A02633-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IFITM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02633-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IFITM1 recombinant protein (Position: M1-Y125).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02633-2 is reactive to IFITM1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
33310 MW
Background of IFITM1
Interferon-induced Transmembrane Protein 1(IFITM1), also called Interferon-induced Protein 17(IFI17). IFITM1 activity is required for primordial germ cells(PGCs) transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. It is mapped to Chr.11 and belongs to the family of interferon-induced transmembrane proteins(Ifitm/mil/fragilis), which encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on their pattern of induction in interferon-sensitive and -resistant cell lines and the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02633-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IFITM1 using anti-IFITM1 antibody (A02633-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFITM1 antigen affinity purified polyclonal antibody (Catalog # A02633-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFITM1 at approximately 15 kDa. The expected band size for IFITM1 is at 15 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IFITM1 (Source: Uniprot.org, NCBI)
Gene Name
IFITM1
Full Name
Interferon-induced transmembrane protein 1
Weight
33310 MW
Superfamily
CD225/Dispanin family
Alternative Names
Cadherin-5; Vascular endothelial cadherin; VE-cadherin; CD144; Cdh5 IFITM1 9-27, CD225, DSPA2a, IFI17, LEU13 interferon induced transmembrane protein 1 interferon-induced transmembrane protein 1|dispanin subfamily A member 2a|interferon-induced protein 17|interferon-inducible protein 9-27|leu-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFITM1, check out the IFITM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFITM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IFITM1 Antibody Picoband® (A02633-2)
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