Product Info Summary
SKU: | A07415 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-IFIT5 Antibody
SKU/Catalog Number
A07415
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IFIT5 Antibody (Catalog # A07415). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFIT5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07415)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human IFIT5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07415 is reactive to IFIT5 in Human
Reconstitution
Calculated molecular weight
55847 MW
Background of IFIT5
Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.
Rogers J., Nature 429:375-381(2004).
Venter J.C., Submitted (SEP-2005).
Bennett K.L., Submitted (OCT-2008).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07415 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using IFIT5 antibody A07415.
Protein Target Info & Infographic
Gene/Protein Information For IFIT5 (Source: Uniprot.org, NCBI)
Gene Name
IFIT5
Full Name
Interferon-induced protein with tetratricopeptide repeats 5
Weight
55847 MW
Superfamily
IFIT family
Alternative Names
Interferon-induced protein with tetratricopeptide repeats 5;IFIT-5;Interferon-induced 58 kDa protein;Retinoic acid- and interferon-inducible 58 kDa protein;P58;IFIT5;ISG58, RI58; IFIT5 ISG58, P58, RI58 interferon induced protein with tetratricopeptide repeats 5 interferon-induced protein with tetratricopeptide repeats 5|IFIT-5|interferon-induced 58 kDa protein|retinoic acid- and interferon-inducible 58 kDa protein|retinoic acid- and interferon-inducible protein (58kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFIT5, check out the IFIT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFIT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IFIT5 Antibody (A07415)
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No publications found for A07415
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-IFIT5 Antibody
Question
We are currently using anti-IFIT5 antibody A07415 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The anti-IFIT5 antibody (A07415) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-05
Question
I see that the anti-IFIT5 antibody A07415 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-IFIT5 antibody A07415. Let me know if you need anything else.
A. Bhatt
Verified customer
Asked: 2018-04-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-23
Question
Do you have a BSA free version of anti-IFIT5 antibody A07415 available?
M. Evans
Verified customer
Asked: 2017-06-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IFIT5 antibody A07415 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-06
Question
Our lab want to know about to test anti-IFIT5 antibody A07415 on human amygdala for research purposes, then I may be interested in using anti-IFIT5 antibody A07415 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Williams
Verified customer
Asked: 2013-07-11
Answer
The products we sell, including anti-IFIT5 antibody A07415, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-11