Product Info Summary
SKU: | A09544-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IDH3A Antibody Picoband®
SKU/Catalog Number
A09544-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-IDH3A Antibody Picoband® catalog # A09544-3. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IDH3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09544-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
E.coli-derived human PAICS recombinant protein (Position: Q69-L425). Human PAICS shares 95.2% and 96.1% amino acid (aa) sequence identity with mouse and rat PAICS, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09544-3 is reactive to IDH3A in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
52588 MW
Background of IDH3A
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3α) is an enzyme that in humans is encoded by the IDH3A gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09544-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
IHC: human esophageal squamous carcinoma tissue, human rectum adenocarcinoma tissue, rat heart tissue
ICC/IF: PC-3 cell
IF: human liver cancer tissue, human intestinal cancer tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
![a09544 3 idh3a primary antibodies wb testing 1 a09544 3 idh3a primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILDR1 antigen affinity purified polyclonal antibody (Catalog # A09544-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILDR1 at approximately 37 kDa. The expected band size for ILDR1 is at 40 kDa.
![a09544 3 idh3a primary antibodies ihc testing 2 a09544 3 idh3a primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09544 3 idh3a primary antibodies ihc testing 3 a09544 3 idh3a primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-ihc-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09544 3 idh3a primary antibodies ihc testing 4 a09544 3 idh3a primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-ihc-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09544 3 idh3a primary antibodies if testing 5 a09544 3 idh3a primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-if-testing-5.jpg)
Click image to see more details
Figure 5. IF analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a09544 3 idh3a primary antibodies if testing 6 a09544 3 idh3a primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-if-testing-6.jpg)
Click image to see more details
Figure 6. IF analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a09544 3 idh3a primary antibodies if testing 7 a09544 3 idh3a primary antibodies if testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-if-testing-7.jpg)
Click image to see more details
Figure 7. IF analysis of ILDR1 using anti-ILDR1 antibody (A09544-3).
ILDR1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ILDR1 Antibody (A09544-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a09544 3 idh3a primary antibodies fcm testing 8 a09544 3 idh3a primary antibodies fcm testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a09544-3-idh3a-primary-antibodies-fcm-testing-8.png)
Click image to see more details
Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ILDR1 antibody (A09544-3).
Overlay histogram showing HepG2 cells stained with A09544-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ILDR1 Antibody (A09544-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For IDH3A (Source: Uniprot.org, NCBI)
Gene Name
IDH3A
Full Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Weight
52588 MW
Superfamily
isocitrate and isopropylmalate dehydrogenases family
Alternative Names
EC 1.1.1; EC 1.1.1.41; H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitrate dehydrogenase 3 (NAD+) alpha; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH IDH3A RP90 isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial|H-IDH alpha|NAD(+)-specific ICDH subunit alpha|NAD(H)-specific isocitrate dehydrogenase alpha subunit|NAD+-specific ICDH|isocitrate dehydrogenase (NAD(+)) 3 alpha|isocitrate dehydrogenase (NAD+) alpha chain|isocitrate dehydrogenase 3 (NAD(+)) alpha|isocitrate dehydrogenase 3 (NAD+) alpha|isocitric dehydrogenase subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDH3A, check out the IDH3A Infographic
![IDH3A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDH3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IDH3A Antibody Picoband® (A09544-3)
Hello CJ!
No publications found for A09544-3
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IDH3A Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IDH3A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question