Product Info Summary
SKU: | M09544 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11]
SKU/Catalog Number
M09544
Size
100 µl
Description
Boster Bio Anti-IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11). Catalog# M09544. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09544)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2F11
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09544 is reactive to IDH3A in Dog, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39.592kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09544 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A.
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Figure from citation: Western blot analysis of IDH3A protein level by using anti-IDH3A antibody in fibroblasts with miR-424 knockdown or overexpression
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
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Flow cytometric analysis of Hela cells
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Flow cytometric analysis of Jurkat cells
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Anti-IDH3A mouse monoclonal antibody (M09544) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A.
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Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (M09544).
Protein Target Info & Infographic
Gene/Protein Information For IDH3A (Source: Uniprot.org, NCBI)
Gene Name
IDH3A
Full Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Weight
39.592kDa
Superfamily
isocitrate and isopropylmalate dehydrogenases family
Alternative Names
EC 1.1.1; EC 1.1.1.41; H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitrate dehydrogenase 3 (NAD+) alpha; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH IDH3A RP90 isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial|H-IDH alpha|NAD(+)-specific ICDH subunit alpha|NAD(H)-specific isocitrate dehydrogenase alpha subunit|NAD+-specific ICDH|isocitrate dehydrogenase (NAD(+)) 3 alpha|isocitrate dehydrogenase (NAD+) alpha chain|isocitrate dehydrogenase 3 (NAD(+)) alpha|isocitrate dehydrogenase 3 (NAD+) alpha|isocitric dehydrogenase subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDH3A, check out the IDH3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDH3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11] (M09544)
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