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Product Info Summary
| SKU: | RP1026 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-survivin/BIRC5 Antibody Picoband®
SKU/Catalog Number
RP1026
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-survivin/BIRC5 Antibody catalog # RP1026. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-survivin/BIRC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1026)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Survivin recombinant protein (Position: M1-D142).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1026 is reactive to BIRC5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
16389 MW
Background of Survivin
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1026 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human 293T whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Click image to see more details
Figure 2. IHC analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026).
Survivin/BIRC5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/BIRC5 Antibody (RP1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For BIRC5 (Source: Uniprot.org, NCBI)
Gene Name
BIRC5
Full Name
Baculoviral IAP repeat-containing protein 5
Weight
16389 MW
Superfamily
IAP family
Alternative Names
Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;BIRC5;API4, IAP4; BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BIRC5, check out the BIRC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BIRC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-survivin/BIRC5 Antibody Picoband® (RP1026)
Hello CJ!
RP1026 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Induction of Apoptosis by Costunolide in Bladder Cancer Cells is Mediated through ROS Generation and Mitochondrial Dysfunction
Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China
Effect of 5-aminolevulinic acid photodynamic therapy on the expression of apoptosis inhibitors Bcl-2 and Survivin in keratinocytes of condyloma acuminatum
Increased survivin expression and its association with clinical parameters of congenital choledochal cysts
Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
Radiosensitivity of human colon cancer cell enhanced by immunoliposomal docetaxel
Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...
Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-survivin/BIRC5 Antibody Picoband®
Question
We have been able to see staining in human myeloid leukemia cell. What should we do? Is anti-survivin/BIRC5 antibody supposed to stain myeloid leukemia cell positively?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Based on literature myeloid leukemia cell does express BIRC5. Based on Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2020-04-27
Question
Would RP1026 anti-survivin/BIRC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
As indicated on the product datasheet, RP1026 anti-survivin/BIRC5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-17
Question
I was wanting to use your anti-survivin/BIRC5 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, RP1026 anti-survivin/BIRC5 antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Does anti-survivin/BIRC5 antibody RP1026 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of lung, BIRC5 is highly expressed in lung. So, it is likely that anti-survivin/BIRC5 antibody RP1026 will work for WB with lung.
Boster Scientific Support
Answered: 2020-03-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-survivin/BIRC5 antibody RP1026. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-02
Question
We need using your anti-survivin/BIRC5 antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on human hek293 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
I appreciate your inquiry. This RP1026 anti-survivin/BIRC5 antibody is tested on human hek293 whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-survivin/BIRC5 antibody RP1026 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The anti-survivin/BIRC5 antibody (RP1026) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-22
Question
Please see the WB image, lot number and protocol we used for lung using anti-survivin/BIRC5 antibody RP1026. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-07
Question
Does anti-survivin/BIRC5 antibody RP1026 work on bovine IHC with mammary gland muscle?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
Our lab technicians have not validated anti-survivin/BIRC5 antibody RP1026 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-survivin/BIRC5 antibody RP1026 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine mammary gland muscle in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-09
Question
I would like to test anti-survivin/BIRC5 antibody RP1026 on human lung for research purposes, then I may be interested in using anti-survivin/BIRC5 antibody RP1026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
The products we sell, including anti-survivin/BIRC5 antibody RP1026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-27
Question
Is a blocking peptide available for product anti-survivin/BIRC5 antibody (RP1026)?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
We do provide the blocking peptide for product anti-survivin/BIRC5 antibody (RP1026). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-19
Question
Can you help my question with product RP1026, anti-survivin/BIRC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1026 anti-survivin/BIRC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-30
Question
Is this RP1026 anti-survivin/BIRC5 antibody reactive to the isotypes of BIRC5?
K. Walker
Verified customer
Asked: 2016-09-01
Answer
The immunogen of RP1026 anti-survivin/BIRC5 antibody is E. coli-derived human Survivin recombinant protein(Position: M1-D142). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-01
Question
Do you have a BSA free version of anti-survivin/BIRC5 antibody RP1026 available?
P. Anderson
Verified customer
Asked: 2016-04-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-survivin/BIRC5 antibody RP1026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-04-08
Question
Our lab used your anti-survivin/BIRC5 antibody for IHC on myeloid leukemia cell in a previous project. I am using human, and We intend to use the antibody for WB next. My lab would like examining myeloid leukemia cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
K. Jones
Verified customer
Asked: 2015-01-02
Answer
I looked at the website and datasheets of our anti-survivin/BIRC5 antibody and I see that RP1026 has been tested on human in both IHC and WB. Thus RP1026 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-02
Question
By saying the recombinant human survivin protein, do you mean that some specific tag was used which increased the overall MW weight to 34kd? We observed 34kd band size with the anti-survivin antibody with the mouse liver lysate, the reason we do not currently know. We surmise it as a dimeric form of the survivin. Thanks for your input.
Verified Customer
Verified customer
Asked: 2014-12-09
Answer
The 34kd of the recombinant protein is the dimeric form of the survivin. Please see the updated image wiht bands at 16 kD on the product page. https://www.bosterbio.com/anti-human-survivin-antibody-rp1026-boster.html?options=cart
Boster Scientific Support
Answered: 2014-12-09
Question
Our team were content with the WB result of your anti-survivin/BIRC5 antibody. However we have been able to see positive staining in mammary gland muscle cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?
M. Mitchell
Verified customer
Asked: 2014-01-15
Answer
Based on literature, mammary gland muscle does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2014-01-15
Question
I see that the anti-survivin/BIRC5 antibody RP1026 works with WB, what is the protocol used to produce the result images on the product page?
J. Taylor
Verified customer
Asked: 2013-11-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-11-01
