Click image to see more details
Product Info Summary
| SKU: | PB9104 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cyclin B1/CCNB1 Antibody Picoband®
SKU/Catalog Number
PB9104
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin B1/CCNB1 Antibody Picoband® catalog # PB9104. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1/CCNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9104)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9104 is reactive to CCNB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
48337 MW
Background of Cyclin B1
CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9104 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: HELA whole cell, 293T whole cell, MCF-7 whole cell, COLO320 whole cell
ICC/IF: A431 cell
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (PB9104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: HELA whole cell lysate,
lane 2: 293T whole cell lysate,
lane 3: MCF-7 whole cell lysate,
lane 4: COLO320 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # PB9104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 56KD.
Click image to see more details
Figure 3. Flow Cytometry analysis of THP-1 cells using anti-CCNB1 antibody (PB9104).
Overlay histogram showing THP-1 cells stained with PB9104 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB1 Antibody (PB9104,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 2. IF analysis of CCNB1 using anti-CCNB1 antibody (PB9104).
CCNB1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCNB1 Antibody (PB9104) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48337 MW
Superfamily
cyclin family
Alternative Names
G2/mitotic-specific cyclin-B1;CCNB1;CCNB; CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1/CCNB1 Antibody Picoband® (PB9104)
Hello CJ!
PB9104 has been cited in 20 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells
Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide
Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells
LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling
Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum
Guo X, Guo N, Zhao J, Cai Y. Oncol Rep. 2017 Sep;38(3):1442-1450. doi: 10.3892/or.2017.5829. Epub 2017 Jul 18. Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells
Ren Y, Wang Q, Shi L, Yue W, Zhang C, Lei F. Anim Reprod Sci. 2011 Feb;123(3-4):187-91. Doi: 10.1016/J.Anireprosci.2011.01.002. Epub 2011 Jan 14. Effects Of Maternal And Dietary Selenium (Se-Enriched Yeast) On The Expression Of P34(Cdc2) And Cycli...
Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cyclin B1/CCNB1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cyclin B1/CCNB1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Cyclin B1/CCNB1 Antibody Picoband®
Question
I am interested in using your anti-Cyclin B1/CCNB1 antibody for mitotic cell cycle phase transition studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We appreciate your inquiry. This PB9104 anti-Cyclin B1/CCNB1 antibody is tested on hela whole cell lysate, 293t whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-13
