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Product Info Summary
| SKU: | A01879-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human PDCD10 DyLight® 488 conjugated Antibody
SKU/Catalog Number
A01879-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human PDCD10 DyLight® 488 conjugated Antibody catalog # A01879-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human PDCD10 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01879-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDCD10 recombinant protein (Position: M1-A212).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01879-Dyl488 is reactive to PDCD10 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
24.702kDa
Background of PDCD10
Programmed cell death protein 10 is a protein that in humans is encoded by the PDCD10 gene. This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01879-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: K562 cell
Validation Images & Assay Conditions
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1. Flow Cytometry analysis of K562 cells using anti-Human PDCD10 antibody (A01879-Dyl488).
Overlay histogram showing K562 cells stained with A01879-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human PDCD10 Antibody (A01879-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PDCD10 (Source: Uniprot.org, NCBI)
Gene Name
PDCD10
Full Name
Programmed cell death protein 10
Weight
24.702kDa
Superfamily
PDCD10 family
Alternative Names
Programmed cell death protein 10; Cerebral cavernous malformations 3 protein; TF-1 cell apoptosis-related protein 15; PDCD10; CCM3; TFAR15 PDCD10 CCM3, TFAR15 programmed cell death 10 programmed cell death protein 10|TF-1 cell apoptosis-related protein 15|apoptosis-related protein 15|cerebral cavernous malformations 3 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD10, check out the PDCD10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human PDCD10 DyLight® 488 conjugated Antibody (A01879-Dyl488)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Human PDCD10 DyLight® 488 conjugated Antibody
Question
For this item: A01879-Dyl488, is the epitope intracellular or extracellular? What is the positive control for this antibody?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The epitope is intracellular. Our lab technicians fix and permeabilize cells samples for the FCM test. K562 cells is suggested as a positive control for this antibody.
Boster Scientific Support
Answered: 2019-11-05
