Click image to see more details
Product Info Summary
| SKU: | M12334 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127
View all Lambda Light Chain Antibodies
SKU/Catalog Number
M12334
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (Catalog # M12334). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12334)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM127
Isotype
Rabbit IgG
Immunogen
Human IgG
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M12334 is reactive to IGLC1 in Human
Reconstitution
Calculated molecular weight
11348 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12334 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.05ug/mL – 0.2ug/mL
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 0.2ug/mL-2ug/mL.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonal antibody (Clone RM127).
Click image to see more details
Figure 2. ELISA result showing specificity
ELISA of human immunoglobulins shows RM127 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
Figure 3. ELISA result showing specificity
A titer ELISA using RM127. The plate was coated with different amounts of human IgG3. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
Figure 4. ELISA result showing specificity
ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGLC1 (Source: Uniprot.org, NCBI)
Gene Name
IGLC1
Full Name
Immunoglobulin lambda constant 1
Weight
11348 MW
Alternative Names
Immunoglobulin lambda constant 1 ;Ig lambda chain C region MGC ;Ig lambda-1 chain C region ;IGLC1 ; IGLC1 IGLC immunoglobulin lambda constant 1 C1 segment|immunoglobulin lambda constant 1 (Mcg marker)|immunoglobulin lambda constant region 1 (Mcg marker)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGLC1, check out the IGLC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGLC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (M12334)
Hello CJ!
No publications found for M12334
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127
Question
We are currently using anti-Human Lambda Light Chain Rabbit Monoclonal antibody, Clone#RM127 M12334 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
J. Jones
Verified customer
Asked: 2019-04-16
Answer
The anti-Human Lambda Light Chain Rabbit Monoclonal antibody, Clone#RM127 (M12334) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-16
