Click image to see more details
Product Info Summary
| SKU: | M00254-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-Human Ki-67 Monoclonal Antibody
View all Ki67/MKI67 Antibodies
SKU/Catalog Number
M00254-2
Size
100ul
Description
Boster Bio Anti-Human Ki-67 Monoclonal Antibody catalog # M00254-2. Tested in IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ki-67 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-2)
Host
Mouse
Contents
Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol, 5mM azide
Clonality
Monoclonal
Immunogen
Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00254-2 is reactive to MKI67 in Human
Reconstitution
Calculated molecular weight
358694 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00254-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-5,000. IF 1:2,000-5,000
Validation Images & Assay Conditions
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Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, M00254-2, dilution 1:2,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.
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High magnification confocal immunofluorescence image of M00254-2 stained HeLa cells grown in tissue culture, in green. The nuclei of rapidly dividing cells have clumps of Ki-67 associated with nucleoli, while nearby quiescent cells express little or no Ki-67. Cells are counterstained with other in red which reveals the nuclear lamina, and DAPI reveals nuclear DNA in blue.
Protein Target Info & Infographic
Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)
Gene Name
MKI67
Full Name
Proliferation marker protein Ki-67
Weight
358694 MW
Alternative Names
Proliferation marker protein Ki-67 ;Antigen identified by monoclonal antibody Ki-67 ;Antigen KI-67 ;Antigen Ki67 ;MKI67 ; MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67| Ki67| by Ki-67|proliferation-related Ki-67 |protein phosphatase 1, regulatory subunit 105
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Ki-67 Monoclonal Antibody (M00254-2)
Hello CJ!
M00254-2 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
M00254-2 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1
WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects
Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo
Soy isoflavone extracts stimulate the growth of nude mouse xenografts bearing estrogen-dependent human breast cancer cells (MCF-7)
Pan Z, Tian Y, Zhang B, Zhang X, Shi H, Liang Z, Wu P, Li R, You B, Yang L, Mao F, Qian H, Xu W. Int J Oncol. 2017 Oct;51(4):1055-1066. doi: 10.3892/ijo.2017.4101. Epub 2017 Aug 23. YAP signaling in gastric cancer-derived mesenchymal stem cells is...
Cheng Z, Wang Hz, Li X, Wu Z, Han Y, Li Y, Chen G, Xie X, Huang Y, Du Z, Zhou Y. J Exp Clin Cancer Res. 2015 Mar 26;34:27. Doi: 10.1186/S13046-015-0142-9. Microrna-184 Inhibits Cell Proliferation And Invasion, And Specifically Targets Tnfaip2 In G...
Zhao Xy, Chen L, Li Yh, Xu Q. World J Gastroenterol. 2007 Dec 28;13(48):6558-61. Plexina1 Expression In Gastric Carcinoma And Its Relationship With Tumor Angiogenesis And Proliferation.
Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...
Yi M, Yang J, Chen X, Li J, Li X, Wang L, Tan Y, Xiong W, Zhou M, Mccarthy Jb, Li G, Xiang B, Xie H. J Cell Physiol. 2011 Sep;226(9):2360-9. Doi: 10.1002/Jcp.22568. Rassf1A Suppresses Melanoma Development By Modulating Apoptosis And Cell-Cycle Pro...
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