Anti-EPO/Erythropoietin Antibody

Product Name

Anti-EPO/Erythropoietin Antibody

View all Erythropoietin/EPO Antibodies

SKU/Catalog Number

RP1004

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EPO/Erythropoietin Antibody catalog # RP1004. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EPO/Erythropoietin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1004)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human EPO recombinant protein (Position: M1-R166).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1004 is reactive to EPO in Human

Applications

RP1004 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

21307 MW

Background of Erythropoietin/EPO

EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22.1. It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Anti-human EPO antibody, RP1004, Western blotting
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2

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Anti-human EPO antibody, RP1004, IHC(P)
IHC(P): Human Placenta Tissue

Gene/Protein Information For EPO (Source: Uniprot.org, NCBI)

Alternative Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin

For more info on EPO, check out the EPO Infographic

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In this infographic, you will see the following information for EPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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