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Product Info Summary
| SKU: | FC02236-PerCP |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated
SKU/Catalog Number
FC02236-PerCP
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated (Catalog# FC02236-PerCP). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02236-PerCP)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT8
Isotype
IgG2a,k
Immunogen
Human peripheral blood T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC02236-PerCP is reactive to Cd8a in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
27456 MW
Background of CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC02236-PerCP is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated human PBMCs stained with PerCP conjugated anti-human CD8 (clone OKT-8, red histogram). PBMCs stained with RPC5.4 IgG2a PerCP isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)
Gene Name
Cd8a
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
27456 MW
Alternative Names
T-cell surface glycoprotein CD8 alpha chain;T-cell surface glycoprotein Lyt-2;CD8a;Cd8a;Lyt-2, Lyt2; CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd8a, check out the Cd8a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated (FC02236-PerCP)
Hello CJ!
FC02236-PerCP has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Anti-human CD8 Cd8a Monoclonal Antibody PerCP Conjugated, Flow Validated
Question
Does FC02236-PerCP anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, FC02236-PerCP anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated (FC02236-PerCP)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
We do provide the blocking peptide for product anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated (FC02236-PerCP). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-10
Question
I see that the anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-29
Question
Does anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP work for Flow Cytometry with thymus?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP will work for Flow Cytometry with thymus.
Boster Scientific Support
Answered: 2019-07-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-18
Question
Can you help my question with product FC02236-PerCP, anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC02236-PerCP anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-04
Question
Do you have a BSA free version of anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP available?
V. Lewis
Verified customer
Asked: 2017-07-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-26
Question
I was wanting to use to test anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP on human thymus for research purposes, then I may be interested in using anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Zhao
Verified customer
Asked: 2014-08-27
Answer
The products we sell, including anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-27
Question
I have attached the WB image, lot number and protocol we used for thymus using anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated FC02236-PerCP. Please let me know if you require anything else.
L. Wu
Verified customer
Asked: 2014-08-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-13
Question
I was wanting to use your anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated for Flow Cytometry for human thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thymus identification?
D. Johnson
Verified customer
Asked: 2013-03-07
Answer
You can see on the product datasheet, FC02236-PerCP anti-human CD8 Monoclonal antibody PerCP Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-07
