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Product Info Summary
| SKU: | FC09548 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD57 B3GAT1 Monoclonal Antibody Unconjugated, Flow Validated
View all beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibodies
SKU/Catalog Number
FC09548
Size
100ug, 500ug
Form
Liquid
Description
Boster Bio Anti-human CD57 B3GAT1 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC09548). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD57 B3GAT1 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC09548)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: HNK-1
Isotype
IgM,k
Immunogen
Animals were immunized with a membrane extract of the human lymphoblastoid cell line HSB-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC09548 is reactive to B3GAT1 in Human
Reconstitution
Calculated molecular weight
38256 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC09548 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs were stained with purified mouse anti-human CD57 (clone HNK-1) followed by staining with goat anti-mouse IgG (GMI) Alexa Fluor 488 along with mouse anti-human CD3 APC (right panel). Lymphocytes gated PBMCs stained with goat anti-mouse IgG (GMI) Alexa Fluor 488 along with mouse anti-human CD3 APC (left panel).
Protein Target Info & Infographic
Gene/Protein Information For B3GAT1 (Source: Uniprot.org, NCBI)
Gene Name
B3GAT1
Full Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Weight
38256 MW
Superfamily
glycosyltransferase 43 family
Alternative Names
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1;2.4.1.135;Beta-1,3-glucuronyltransferase 1;Glucuronosyltransferase P;GlcAT-P;UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase;GlcUAT-P;B3GAT1;GLCATP; B3GAT1 CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1 beta-1,3-glucuronyltransferase 1 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1|LEU7 |UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase|glucuronosyltransferase P
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B3GAT1, check out the B3GAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B3GAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD57 B3GAT1 Monoclonal Antibody Unconjugated, Flow Validated (FC09548)
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No publications found for FC09548
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-human CD57 B3GAT1 Monoclonal Antibody Unconjugated, Flow Validated
Question
Would anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 work for Flow Cytometry with c1 segment of cervical spinal cord?
K. Banerjee
Verified customer
Asked: 2020-04-27
Answer
According to the expression profile of c1 segment of cervical spinal cord, B3GAT1 is highly expressed in c1 segment of cervical spinal cord. So, it is likely that anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 will work for Flow Cytometry with c1 segment of cervical spinal cord.
Boster Scientific Support
Answered: 2020-04-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-04
Question
Is there a BSA free version of anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 available?
K. Wu
Verified customer
Asked: 2019-09-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-18
Question
Can you help my question with product FC09548, anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC09548 anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-21
Question
Will anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 work on bovine Flow Cytometry with brain?
D. Jha
Verified customer
Asked: 2018-01-29
Answer
Our lab technicians have not validated anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated FC09548 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-29
Question
Does FC09548 anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Yang
Verified customer
Asked: 2017-03-09
Answer
It shows on the product datasheet, FC09548 anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-09
Question
Is a blocking peptide available for product anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated (FC09548)?
L. Thomas
Verified customer
Asked: 2014-02-05
Answer
We do provide the blocking peptide for product anti-human CD57 Monoclonal antibody Unconjugated, Flow Validated (FC09548). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-02-05
