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Product Info Summary
| SKU: | FC00555-PerCP-Cy5.5 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD45 PTPRC Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
SKU/Catalog Number
FC00555-PerCP-Cy5.5
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD45 PTPRC Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00555-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD45 PTPRC Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00555-PerCP-Cy5.5)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: F10-89-4
Isotype
IgG2a,k
Immunogen
Human T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
FC00555-PerCP-Cy5.5 is reactive to PTPRC in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
147254 MW
Background of PTPRC
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00555-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained withPerCP-Cyanine5.5 conjugated antihumanCD45 (clone F10-89-4, redhistogram). PBMCs lymphocytesstained with IgG2a PerCP-Cyanine5.5 isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD45 PTPRC Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00555-PerCP-Cy5.5)
Hello CJ!
FC00555-PerCP-Cy5.5 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis
Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-human CD45 PTPRC Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
Question
Our lab want to know about to test anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00555-PerCP-Cy5.5 on human synovium for research purposes, then I may be interested in using anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00555-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The products we sell, including anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00555-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-01
Question
I was wanting to use your anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human synovium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human synovium identification?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
You can see on the product datasheet, FC00555-PerCP-Cy5.5 anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human synovium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-18
Question
Is this FC00555-PerCP-Cy5.5 anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated reactive to the isotypes of PTPRC?
B. Li
Verified customer
Asked: 2017-03-28
Answer
The immunogen of FC00555-PerCP-Cy5.5 anti-human CD45 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated is Human T lymphocytes . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-03-28
