Click image to see more details
Product Info Summary
| SKU: | FC01450-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD32 FCGR2A Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC01450-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD32 FCGR2A Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC01450-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD32 FCGR2A Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01450-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: IV.3
Isotype
IgG2b,k
Immunogen
K-562 human erythromyeloblastoid leukemia cell line
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01450-PE is reactive to FCGR2A in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
35001 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01450-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Monocytes gated PBMCs stained with PE conjugated anti-human CD32 (clone IV.3) (red histogram). Monocytes gated PBMCs stained with anti-human clone IgG1 MOPC- 31 PE (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For FCGR2A (Source: Uniprot.org, NCBI)
Gene Name
FCGR2A
Full Name
Low affinity immunoglobulin gamma Fc region receptor II-a
Weight
35001 MW
Alternative Names
Low affinity immunoglobulin gamma Fc region receptor II-a;IgG Fc receptor II-a;CDw32;Fc-gamma RII-a;Fc-gamma-RIIa;FcRII-a;CD32;FCGR2A;CD32, FCG2, FCGR2A1, IGFR2; FCGR2A CD32, CD32A, CDw32, FCG2, FCGR21, FcGR, IGFR2, FCGR2A Fc fragment of IgG receptor IIa low affinity immunoglobulin gamma Fc region receptor II-a|Fc fragment of IgG, low affinity IIa, receptor (CD32)|Fc gamma receptor RIIa3|Immunoglobulin G Fc receptor II|fc-gamma-RIIa|fcRII-a|igG Fc receptor II-a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR2A, check out the FCGR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD32 FCGR2A Monoclonal Antibody PE Conjugated, Flow Validated (FC01450-PE)
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No publications found for FC01450-PE
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-human CD32 FCGR2A Monoclonal Antibody PE Conjugated, Flow Validated
Question
Is a blocking peptide available for product anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated (FC01450-PE)?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do provide the blocking peptide for product anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated (FC01450-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-29
Question
Would FC01450-PE anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
You can see on the product datasheet, FC01450-PE anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-26
Question
I have attached the WB image, lot number and protocol we used for blood using anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated FC01450-PE. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-09
Question
Does anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated FC01450-PE work for Flow Cytometry with blood?
B. Carter
Verified customer
Asked: 2013-07-17
Answer
According to the expression profile of blood, FCGR2A is highly expressed in blood. So, it is likely that anti-human CD32 Monoclonal antibody PE Conjugated, Flow Validated FC01450-PE will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2013-07-17
