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Product Info Summary
| SKU: | FC00154 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD19 Monoclonal Antibody Unconjugated, Flow Validated
SKU/Catalog Number
FC00154
Size
100ug, 500ug
Form
Liquid
Description
Boster Bio Anti-human CD19 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC00154). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD19 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00154)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 4G7
Isotype
IgG1,k
Immunogen
Human CCL (chronic lymphocytic leukemia) cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00154 is reactive to CD19 in Human
Reconstitution
Calculated molecular weight
61128 MW
Background of CD19
B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00154 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Human PBMCs stained with purified anti-human CD19 (clone 4G7) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488. PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61128 MW
Alternative Names
B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19;CD19; CD19 B4, CVID3 CD19 molecule B-lymphocyte CD19|B-lymphocyte surface B4|T-cell surface Leu-12|differentiation CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD19 Monoclonal Antibody Unconjugated, Flow Validated (FC00154)
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No publications found for FC00154
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Customer Q&As
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10 Customer Q&As for Anti-human CD19 Monoclonal Antibody Unconjugated, Flow Validated
Question
I have a question about product FC00154, anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00154 anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
As indicated on the product datasheet, FC00154 anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Does anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 work for Flow Cytometry with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of embryonic kidney, CD19 is highly expressed in embryonic kidney. So, it is likely that anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 will work for Flow Cytometry with embryonic kidney.
Boster Scientific Support
Answered: 2019-07-04
Question
Is a blocking peptide available for product anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated (FC00154)?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
We do provide the blocking peptide for product anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated (FC00154). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-06
Question
We need to test anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 on human embryonic kidney for research purposes, then I may be interested in using anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Parker
Verified customer
Asked: 2018-08-22
Answer
The products we sell, including anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-31
Question
Does FC00154 anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
As indicated on the product datasheet, FC00154 anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-09
Question
Is there a BSA free version of anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 available?
D. Evans
Verified customer
Asked: 2014-08-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-14
Question
I have attached the WB image, lot number and protocol we used for embryonic kidney using anti-human CD19 Monoclonal antibody Unconjugated, Flow Validated FC00154. Please let me know if you require anything else.
J. Miller
Verified customer
Asked: 2013-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-09
