Product Info Summary
SKU: | FC00357-PE |
---|---|
Size: | 100 tests, 200 tests |
Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD11c ITGAX Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC00357-PE
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD11c ITGAX Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC00357-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD11c ITGAX Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00357-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 3.9
Isotype
IgG1,k
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00357-PE is reactive to ITGAX in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
127829 MW
Background of CD11c
CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00357-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Monocytes gated PBMCs stained with PE conjugated anti-human CD11c (clone 3.9) (red histogram). Monocytes gated PBMCs stained with PE conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For ITGAX (Source: Uniprot.org, NCBI)
Gene Name
ITGAX
Full Name
Integrin alpha-X
Weight
127829 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-X;CD11 antigen-like family member C;Leu M5;Leukocyte adhesion glycoprotein p150,95 alpha chain;Leukocyte adhesion receptor p150,95;CD11c;ITGAX;CD11C; ITGAX CD11C, SLEB6 integrin subunit alpha X integrin alpha-X|CD11 -like family member C|complement component 3 receptor 4 subunit|integrin alpha X|integrin, alpha X ( CD11C (p150), alpha polypeptide)|integrin, alpha X (complement component 3 receptor 4 subunit)|leu M5, alpha subunit|leukocyte adhesion glycoprotein p150,95 alpha chain|leukocyte adhesion receptor p150,95|leukocyte surface p150,95, alpha subunit|myeloid membrane , alpha subunit|p150 95 integrin alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAX, check out the ITGAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD11c ITGAX Monoclonal Antibody PE Conjugated, Flow Validated (FC00357-PE)
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No publications found for FC00357-PE
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Customer Q&As
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3 Customer Q&As for Anti-Human CD11c ITGAX Monoclonal Antibody PE Conjugated, Flow Validated
Question
you antibody to test anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated FC00357-PE on human blood for research purposes, then I may be interested in using anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated FC00357-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Gonzalez
Verified customer
Asked: 2020-03-02
Answer
The products we sell, including anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated FC00357-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-02
Question
I was wanting to use your anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
As indicated on the product datasheet, FC00357-PE anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-26
Question
Is this FC00357-PE anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated reactive to the isotypes of ITGAX?
A. Jha
Verified customer
Asked: 2013-12-24
Answer
The immunogen of FC00357-PE anti-Human CD11c Monoclonal antibody PE Conjugated, Flow Validated is Rheumatoid synovial fluid cells and fibronectin purified human monocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-12-24