Click image to see more details
Product Info Summary
| SKU: | A00357-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD11c/ITGAX Antibody Picoband®
SKU/Catalog Number
A00357-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD11c/ITGAX Antibody Picoband® catalog # A00357-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11c/ITGAX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00357-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD11c recombinant protein (Position: S161-T342).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00357-1 is reactive to ITGAX in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
132494 MW
Background of CD11c
CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00357-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse spleen tissue, mouse thymus tissue
IHC: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD11c using anti-CD11c antibody (A00357-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysate,
Lane 2: mouse thymus tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11c antigen affinity purified polyclonal antibody (Catalog # A00357-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11c at approximately 150KD. The expected band size for CD11c is at 128KD.
Click image to see more details
Figure 2. IHC analysis of CD11c using anti-CD11c antibody (A00357-1).
CD11c was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD11c Antibody (A00357-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ITGAX (Source: Uniprot.org, NCBI)
Gene Name
ITGAX
Full Name
Integrin alpha-X
Weight
132494 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-X; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD11c; ITGAX; CD11C ITGAX CD11C, SLEB6 integrin subunit alpha X integrin alpha-X|CD11 -like family member C|complement component 3 receptor 4 subunit|integrin alpha X|integrin, alpha X ( CD11C (p150), alpha polypeptide)|integrin, alpha X (complement component 3 receptor 4 subunit)|leu M5, alpha subunit|leukocyte adhesion glycoprotein p150,95 alpha chain|leukocyte adhesion receptor p150,95|leukocyte surface p150,95, alpha subunit|myeloid membrane , alpha subunit|p150 95 integrin alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAX, check out the ITGAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11c/ITGAX Antibody Picoband® (A00357-1)
Hello CJ!
A00357-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CD11c/ITGAX Antibody Picoband®
Question
Will A00357-1 anti-CD11c/ITGAX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
As indicated on the product datasheet, A00357-1 anti-CD11c/ITGAX antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
I was wanting to use your anti-CD11c/ITGAX antibody for ELISA for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
It shows on the product datasheet, A00357-1 anti-CD11c/ITGAX antibody has been validated for ELISA, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-19
Question
Would anti-CD11c/ITGAX antibody A00357-1 work on horse WB with blood?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not validated anti-CD11c/ITGAX antibody A00357-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD11c/ITGAX antibody A00357-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD11c/ITGAX antibody A00357-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
I see that the anti-CD11c/ITGAX antibody A00357-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-11
Question
We have observed staining in mouse liver. Any tips? Is anti-CD11c/ITGAX antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
From literature liver does express ITGAX. From Uniprot.org, ITGAX is expressed in blood, liver, among other tissues. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-07-29
Question
My question regarding product A00357-1, anti-CD11c/ITGAX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00357-1 anti-CD11c/ITGAX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
We bought anti-CD11c/ITGAX antibody for IHC on liver a few months ago. I am using human, and We want to use the antibody for ELISA next. My lab would like examining liver as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
I looked at the website and datasheets of our anti-CD11c/ITGAX antibody and it appears that A00357-1 has been tested on human in both IHC and ELISA. Thus A00357-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-26
Question
Our lab were satisfied with the WB result of your anti-CD11c/ITGAX antibody. However we have observed positive staining in blood membrane using this antibody. Is that expected? Could you tell me where is ITGAX supposed to be expressed?
J. Li
Verified customer
Asked: 2018-11-05
Answer
According to literature, blood does express ITGAX. Generally ITGAX expresses in membrane. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2018-11-05
Question
Would anti-CD11c/ITGAX antibody A00357-1 work for ELISA with liver?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
According to the expression profile of liver, ITGAX is highly expressed in liver. So, it is likely that anti-CD11c/ITGAX antibody A00357-1 will work for ELISA with liver.
Boster Scientific Support
Answered: 2018-05-25
Question
We need to test anti-CD11c/ITGAX antibody A00357-1 on mouse liver for research purposes, then I may be interested in using anti-CD11c/ITGAX antibody A00357-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The products we sell, including anti-CD11c/ITGAX antibody A00357-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-07
Question
See attached the WB image, lot number and protocol we used for liver using anti-CD11c/ITGAX antibody A00357-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-16
Question
We are currently using anti-CD11c/ITGAX antibody A00357-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
The anti-CD11c/ITGAX antibody (A00357-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-13
Question
Do you have a BSA free version of anti-CD11c/ITGAX antibody A00357-1 available?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11c/ITGAX antibody A00357-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-15
Question
Is a blocking peptide available for product anti-CD11c/ITGAX antibody (A00357-1)?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
We do provide the blocking peptide for product anti-CD11c/ITGAX antibody (A00357-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-06
Question
I would like using your anti-CD11c/ITGAX antibody for leukocyte migration studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
We appreciate your inquiry. This A00357-1 anti-CD11c/ITGAX antibody is validated on mouse thymus tissue, spleen tissue, tissue lysate, human tonsil tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-26
Question
Is this A00357-1 anti-CD11c/ITGAX antibody reactive to the isotypes of ITGAX?
J. Jones
Verified customer
Asked: 2013-07-18
Answer
The immunogen of A00357-1 anti-CD11c/ITGAX antibody is E. coli-derived human CD11c recombinant protein (Position: S161-T342). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-18
