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Product Info Summary
| SKU: | A09950 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HUCE1 RRP8 Antibody
SKU/Catalog Number
A09950
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HUCE1 RRP8 Antibody (Catalog # A09950). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HUCE1 RRP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09950)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human HUCE1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09950 is reactive to RRP8 in Human
Reconstitution
Calculated molecular weight
50715 MW
Background of HUCE1
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Yazaki M., Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
Ishikawa K., DNA Res. 4:307-313(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09950 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from MCF-7 cells, using HUCE1 antibody A09950.
Protein Target Info & Infographic
Gene/Protein Information For RRP8 (Source: Uniprot.org, NCBI)
Gene Name
RRP8
Full Name
Ribosomal RNA-processing protein 8
Weight
50715 MW
Superfamily
methyltransferase superfamily
Alternative Names
Ribosomal RNA-processing protein 8;2.1.1.-;Cerebral protein 1;Nucleomethylin;RRP8;KIAA0409, NML;hucep-1; RRP8 KIAA0409, NML ribosomal RNA processing 8 ribosomal RNA-processing protein 8|RRP8 methyltransferase homolog|cerebral protein 1|nucleomethylin|ribosomal RNA processing 8, methyltransferase, homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRP8, check out the RRP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HUCE1 RRP8 Antibody (A09950)
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No publications found for A09950
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Customer Q&As
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4 Customer Q&As for Anti-HUCE1 RRP8 Antibody
Question
I was wanting to use your anti-HUCE1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
As indicated on the product datasheet, A09950 anti-HUCE1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-HUCE1 antibody A09950. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
I would like to test anti-HUCE1 antibody A09950 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-HUCE1 antibody A09950 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The products we sell, including anti-HUCE1 antibody A09950, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-07
Question
Does anti-HUCE1 antibody A09950 work on horse WB with lower esophagus muscularis layer?
D. Bhatt
Verified customer
Asked: 2016-03-23
Answer
Our lab technicians have not validated anti-HUCE1 antibody A09950 on horse. You can run a BLAST between horse and the immunogen sequence of anti-HUCE1 antibody A09950 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lower esophagus muscularis layer in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-23
