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Product Info Summary
| SKU: | A08169-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TCP1 eta/CCT7 Antibody Picoband®
SKU/Catalog Number
A08169-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TCP1 eta/CCT7 Antibody Picoband® catalog # A08169-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCP1 eta/CCT7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08169-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CCT7 recombinant protein (Position: Q30-D307).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08169-2 is reactive to CCT7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.367kDa
Background of TCP1-eta
T-complex protein 1 subunit eta is a protein that in humans is encoded by the CCT7 gene. This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08169-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A549 whole cell, human CACO-2 whole cell, human 293T whole cell, human HepG2 whole cell, human MCF-7 whole cellhuman HEL whole cell, human U251 whole cell, human K562 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
ICC/IF: U20S cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CCT7 using anti-CCT7 antibody (A08169-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
Lane 6: human HEL whole cell lysates,
Lane 7: human U251 whole cell lysates,
Lane 8: human K562 whole cell lysates,
Lane 9: rat C6 whole cell lysates,
Lane 10: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT7 antigen affinity purified polyclonal antibody (Catalog # A08169-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT7 at approximately 59 kDa. The expected band size for CCT7 is at 59 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2).
Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. IF analysis of CCT7 using anti-CCT7 antibody (A08169-2).
CCT7 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT7 Antibody (A08169-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CCT7 (Source: Uniprot.org, NCBI)
Gene Name
CCT7
Full Name
T-complex protein 1 subunit eta
Weight
59.367kDa
Superfamily
TCP-1 chaperonin family
Alternative Names
T-complex protein 1 subunit eta; TCP-1-eta; CCT-eta; HIV-1 Nef-interacting protein; T-complex protein 1 subunit eta, N-terminally processed; CCT7; CCTH; NIP7-1 CCT7 CCTETA, CCTH, NIP7-1, TCP1ETA chaperonin containing TCP1 subunit 7 T-complex protein 1 subunit eta|CCT-eta|HIV-1 Nef interacting protein|TCP-1-eta|chaperonin containing t-complex polypeptide 1, eta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCT7, check out the CCT7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCT7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCP1 eta/CCT7 Antibody Picoband® (A08169-2)
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Customer Q&As
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2 Customer Q&As for Anti-TCP1 eta/CCT7 Antibody Picoband®
Question
We are currently using anti-TCP1 eta/CCT7 antibody A08169-2 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-TCP1 eta/CCT7 antibody (A08169-2) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
Is this A08169-2 anti-TCP1 eta/CCT7 antibody reactive to the isotypes of CCT7?
S. Jones
Verified customer
Asked: 2013-06-18
Answer
The immunogen of A08169-2 anti-TCP1 eta/CCT7 antibody is E. coli-derived human CCT7 recombinant protein (Position: Q30-D307). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-18
