Click image to see more details
Product Info Summary
| SKU: | FC02675-1 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human CD3 Monoclonal Antibody, Flow Validated
SKU/Catalog Number
FC02675-1
Size
100ug, 500ug
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD3 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC02675-1). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD3 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02675-1)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: UCHT1
Isotype
IgG1,k
Immunogen
Thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC02675-1 is reactive to CD3E in Human
Reconstitution
Calculated molecular weight
23147 MW
Background of CD3E
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC02675-1 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
PBMCs lymphocytes stained with purified anti-human CD3 (clone UCHT1) followed by staining with Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). PBMCs lymphocytes stained with mouse IgG1 isotype control followed by staining with Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)
Gene Name
CD3E
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
23147 MW
Alternative Names
T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E; CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e , epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell receptor complex, epsilon subunit of T3|T-cell surface T3/Leu-4 epsilon chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD3E, check out the CD3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD3 Monoclonal Antibody, Flow Validated (FC02675-1)
Hello CJ!
FC02675-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human CD3 Monoclonal Antibody, Flow Validated?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human CD3 Monoclonal Antibody, Flow Validated
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
