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Product Info Summary
| SKU: | A02492-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HSPB8/HSP22 Monoclonal Antibody
View all HSPB8/HSP22 Antibodies
SKU/Catalog Number
A02492-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSPB8/HSP22 Monoclonal Antibody catalog # A02492-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPB8/HSP22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02492-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
2C3
Isotype
IgG1
Immunogen
Recombinant Protein of HSPB8/HSP22
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02492-1 is reactive to HSPB8 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
21604 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02492-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293T with HSPB8/HSP22 Mouse Monoclonal antibody diluted at 1:2000.
Protein Target Info & Infographic
Gene/Protein Information For HSPB8 (Source: Uniprot.org, NCBI)
Gene Name
HSPB8
Full Name
Heat shock protein beta-8
Weight
21604 MW
Superfamily
small heat shock protein (HSP20) family
Alternative Names
Heat shock protein beta-8;HspB8;Alpha-crystallin C chain;E2-induced gene 1 protein;Protein kinase H11;Small stress protein-like protein HSP22;HSPB8;CRYAC, E2IG1, HSP22;PP1629; HSPB8 CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22 heat shock protein family B (small) member 8 heat shock protein beta-8|E2-induced gene 1 protein|alpha-crystallin C chain|heat shock 22kDa protein 8|heat shock 27kDa protein 8|protein kinase H11|small stress protein-like protein HSP22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB8, check out the HSPB8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPB8/HSP22 Monoclonal Antibody (A02492-1)
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No publications found for A02492-1
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Customer Q&As
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7 Customer Q&As for Anti-HSPB8/HSP22 Monoclonal Antibody
Question
Is there a BSA free version of anti-HSPB8/HSP22 Monoclonal antibody A02492-1 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HSPB8/HSP22 Monoclonal antibody A02492-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
I have attached the WB image, lot number and protocol we used for thalamus using anti-HSPB8/HSP22 Monoclonal antibody A02492-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-16
Question
Would A02492-1 anti-HSPB8/HSP22 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
It shows on the product datasheet, A02492-1 anti-HSPB8/HSP22 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-15
Question
Is a blocking peptide available for product anti-HSPB8/HSP22 Monoclonal antibody (A02492-1)?
E. Wu
Verified customer
Asked: 2014-05-14
Answer
We do provide the blocking peptide for product anti-HSPB8/HSP22 Monoclonal antibody (A02492-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-05-14
Question
Is this A02492-1 anti-HSPB8/HSP22 Monoclonal antibody reactive to the isotypes of HSPB8?
T. Li
Verified customer
Asked: 2014-02-07
Answer
The immunogen of A02492-1 anti-HSPB8/HSP22 Monoclonal antibody is Recombinant Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-07
Question
I see that the anti-HSPB8/HSP22 Monoclonal antibody A02492-1 works with WB, what is the protocol used to produce the result images on the product page?
S. Yang
Verified customer
Asked: 2013-10-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-10-07
Question
Would anti-HSPB8/HSP22 Monoclonal antibody A02492-1 work for WB with thalamus?
A. Patel
Verified customer
Asked: 2013-08-01
Answer
According to the expression profile of thalamus, HSPB8 is highly expressed in thalamus. So, it is likely that anti-HSPB8/HSP22 Monoclonal antibody A02492-1 will work for WB with thalamus.
Boster Scientific Support
Answered: 2013-08-01
