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Product Info Summary
| SKU: | M02492-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8)
View all HSPB8/HSP22 Antibodies
SKU/Catalog Number
M02492-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) catalog # M02492-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02492-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
7D8
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02492-2 is reactive to HSPB8 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
21.604kDa
Background of HSPB8/HSP22
Heat shock protein beta-8 is a protein that in humans is encoded by the HSPB8 gene. The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02492-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell,, human T-47D whole cell,, rat RH35 whole cell
ICC/IF: A431 cell
FCM: CACO-2 cell, U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (M02492-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates;
Lane 2: human T-47D whole cell lysates;
Lane 3: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPB8/Hsp22 antigen affinity purified monoclonal antibody (Catalog # M02492-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPB8/Hsp22 at approximately 22KD. The expected band size for HSPB8/Hsp22 is at 22KD.
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Figure 2. Flow Cytometry analysis of CACO-2 cells using anti- HSPB8/Hsp22 antibody (M02492-2).
Overlay histogram showing CACO-2 cells stained with M02492-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HSPB8/Hsp22 Antibody (M02492-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of U20S cells using anti- HSPB8/Hsp22 antibody (M02492-2).
Overlay histogram showing U20S cells stained with M02492-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HSPB8/Hsp22 Antibody (M02492-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. IF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (M02492-2).
HSPB8/Hsp22 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-HSPB8/Hsp22 Antibody (M02492-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HSPB8 (Source: Uniprot.org, NCBI)
Gene Name
HSPB8
Full Name
Heat shock protein beta-8
Weight
21.604kDa
Superfamily
small heat shock protein (HSP20) family
Alternative Names
CMT2L; CRYAC; DHMN 2; DHMN2; E2IG1; H11; HMN 2; HMN2; HMN2A; HSB8; HSPB 8; HspB8 HSPB8 CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22 heat shock protein family B (small) member 8 heat shock protein beta-8|E2-induced gene 1 protein|alpha-crystallin C chain|heat shock 22kDa protein 8|heat shock 27kDa protein 8|protein kinase H11|small stress protein-like protein HSP22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB8, check out the HSPB8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) (M02492-2)
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