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Product Info Summary
| SKU: | MA1050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)
SKU/Catalog Number
MA1050
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
BRM-22
Isotype
Mouse IgG1
Immunogen
HSP70 isolated from bovine brain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1050 is reactive to Hspa1b in Bovine, Human, Mouse, Rabbit, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
70176 MW
Background of HSPA1B
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1050 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat
Positive Control
WB: human MCF-7 whole cell, human 293T whole cell, human A549 whole cell, human U20S whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSP70 using anti-HSP70 antibody (MA1050).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP70 antigen affinity purified monoclonal antibody (Catalog # MA1050) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
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Figure 2. IHC analysis of HSP70 using anti-HSP70 antibody (MA1050).
HSP70 was detected in paraffin-embedded section of human breast cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP70 Antibody (MA1050) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Hspa1b (Source: Uniprot.org, NCBI)
Gene Name
Hspa1b
Full Name
Heat shock 70 kDa protein 1B
Weight
70176 MW
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1; HSPA1B HSP70-1, HSP70-1B, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSX70 heat shock protein family A (Hsp70) member 1B heat shock 70 kDa protein 1B|HSP70-1/HSP70-2|HSP70.1/HSP70.2|Heat shock 70 kDa protein 1|Heat shock 70 kDa protein 1A|heat shock 70 kDa protein 1/2|heat shock 70 kDa protein 1A/1B|heat shock 70 kDa protein 2|heat shock 70kD protein 1B|heat shock 70kDa protein 1B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hspa1b, check out the Hspa1b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hspa1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) (MA1050)
Hello CJ!
MA1050 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle
Wang,D.,Ripley-Gonzalez,J.W.& Hu,Y.Aerobic Physical Training Protects the Rat Brain Against Exercise-Heat Related Oxidative Damage through the Increased Expression of HSP70.Neurophysiology 51,66–71(2019).https://doi.org/10.1007/s11062-019-09794-9
Species: Rat
MA1050 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:400
Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...
Chen S, Zuo X, Yang M, Lu H, Wang N, Wang K, Tu Z, Chen G, Liu M, Liu K, Xiao X. J Leukoc Biol. 2012 Oct;92(4):851-7. Doi: 10.1189/Jlb.0212060. Epub 2012 Jun 29. Severe Multiple Organ Injury In Hsf1 Knockout Mice Induced By Lipopolysaccharide Is A...
Ouyang W, Gao F, Wang L, Xie X, Lei F, Zhou J, Liao Y, Zhong M, Tang J. Oncol Rep. 2010 Oct;24(4):973-9. Thermoseed Hyperthermia Treatment Of Mammary Orthotopic Transplantation Tumors In Rats And Impact On Immune Function.
Xu Xg, Luo Yj, Wu Y, Chen Jz, Xu Th, Gao Xh, He Cd, Geng L, Xiao T, Zhang Yq, Chen Hd, Li Yh. Dermatol Surg. 2011 Aug;37(8):1141-9. Doi: 10.1111/J.1524-4725.2011.02062.X. Epub 2011 Jun 7. Immunohistological Evaluation Of Skin Responses After Treat...
Chen N, Wan Xl, Huang Cx, Wang Wm, Liu H, Wang Hl. Immunobiology. 2014 Nov;219(11):850-8. Doi: 10.1016/J.Imbio.2014.07.012. Epub 2014 Jul 21. Study On The Immune Response To Recombinant Hsp70 Protein From Megalobrama Amblycephala.
Fan Sj, Jiang H, Yang Lj, Liu X, Song J, Pan F. Ann Anat. 2011 Oct 20;193(5):418-24. Doi: 10.1016/J.Aanat.2011.06.001. Epub 2011 Jul 8. Effects Of Adrenergic Agents On Stress-Induced Brain Microstructural And Immunochemical Changes In Adult Male W...
Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.
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