Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody

Product Name

Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody

View all HSP60 Antibodies

SKU/Catalog Number

M01280

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody (Catalog # M01280). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01280)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM253

Isotype

IgG1, kappa

Immunogen

Recombinant human HSP60 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01280 is reactive to HSPD1 in Chicken, Cow, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

61055 MW

Background of HSP60

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01280 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For HSPD1 (Source: Uniprot.org, NCBI)

Alternative Names

60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;Heat shock protein 60;HSP-60;Hsp60;HuCHA60;Mitochondrial matrix protein P1;P60 lymphocyte protein;HSPD1;HSP60; HSPD1 CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13 heat shock protein family D (Hsp60) member 1 60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|P60 lymphocyte protein|chaperonin 60|epididymis secretory sperm binding protein|heat shock 60kDa protein 1 (chaperonin)|heat shock protein 65|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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