Click image to see more details
Product Info Summary
| SKU: | M00411 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-MIF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00411
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MIF Rabbit Monoclonal Antibody catalog # M00411. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00411)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABAG-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MIF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00411 is reactive to MIF in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
12 kDa
Calculated molecular weight
12476 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00411 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
FC 1:50
Positive Control
WB: human THP-1 whole cell, human 293T whole cell, human U2OS whole cell, human HepG2 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MIF using anti-MIF antibody (M00411).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified monoclonal antibody (M00411) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MIF (Source: Uniprot.org, NCBI)
Gene Name
MIF
Full Name
Macrophage migration inhibitory factor
Weight
12476 MW
Superfamily
MIF family
Alternative Names
Macrophage migration inhibitory factor;MIF;5.3.2.1;Glycosylation-inhibiting factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;5.3.3.12;Phenylpyruvate tautomerase;MIF;GLIF, MMIF; MIF GIF, GLIF, MMIF macrophage migration inhibitory factor macrophage migration inhibitory factor|L-dopachrome isomerase|L-dopachrome tautomerase|epididymis secretory sperm binding protein|macrophage migration inhibitory factor (glycosylation-inhibiting factor)|phenylpyruvate tautomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIF, check out the MIF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIF Rabbit Monoclonal Antibody (M00411)
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No publications found for M00411
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Customer Q&As
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16 Customer Q&As for Anti-MIF Rabbit Monoclonal Antibody
Question
We have been able to see staining in mouse left adrenal gland. What should we do? Is anti-MIF Rabbit Monoclonal antibody supposed to stain left adrenal gland positively?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
From what I have seen in literature left adrenal gland does express MIF. From what I have seen in Uniprot.org, MIF is expressed in left adrenal gland, brain, brain, lung, skin uterus, platelet, liver, lens, among other tissues. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2020-04-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MIF Rabbit Monoclonal antibody M00411. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-MIF Rabbit Monoclonal antibody M00411 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-25
Question
Please see the WB image, lot number and protocol we used for brain using anti-MIF Rabbit Monoclonal antibody M00411. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
I am looking for to test anti-MIF Rabbit Monoclonal antibody M00411 on human brain for research purposes, then I may be interested in using anti-MIF Rabbit Monoclonal antibody M00411 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-MIF Rabbit Monoclonal antibody M00411, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
Do you have a BSA free version of anti-MIF Rabbit Monoclonal antibody M00411 available?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MIF Rabbit Monoclonal antibody M00411 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-13
Question
My team were content with the WB result of your anti-MIF Rabbit Monoclonal antibody. However we have seen positive staining in platelet secreted using this antibody. Is that expected? Could you tell me where is MIF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
According to literature, platelet does express MIF. Generally MIF expresses in secreted. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2019-09-10
Question
We are currently using anti-MIF Rabbit Monoclonal antibody M00411 for mouse tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The anti-MIF Rabbit Monoclonal antibody (M00411) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
Does anti-MIF Rabbit Monoclonal antibody M00411 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
According to the expression profile of brain, MIF is highly expressed in brain. So, it is likely that anti-MIF Rabbit Monoclonal antibody M00411 will work for WB with brain.
Boster Scientific Support
Answered: 2019-08-14
Question
Can you help my question with product M00411, anti-MIF Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00411 anti-MIF Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-01
Question
Is this M00411 anti-MIF Rabbit Monoclonal antibody reactive to the isotypes of MIF?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
The immunogen of M00411 anti-MIF Rabbit Monoclonal antibody is A synthesized peptide derived from human MIF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-16
Question
Would M00411 anti-MIF Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Wu
Verified customer
Asked: 2018-02-13
Answer
It shows on the product datasheet, M00411 anti-MIF Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-13
Question
I was wanting to use using your anti-MIF Rabbit Monoclonal antibody for positive regulation of peptidyl-serine phosphorylation studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
We appreciate your inquiry. This M00411 anti-MIF Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for Flow Cytometry, IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-16
Question
We purchased anti-MIF Rabbit Monoclonal antibody for WB on platelet in a previous experiment. I am using mouse, and I plan to use the antibody for Flow Cytometry next. I am interested in examining platelet as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
I have checked the website and datasheets of our anti-MIF Rabbit Monoclonal antibody and it seems that M00411 has been tested on mouse in both WB and Flow Cytometry. Thus M00411 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-05
Question
I was wanting to use your anti-MIF Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
A. Moore
Verified customer
Asked: 2015-02-23
Answer
It shows on the product datasheet, M00411 anti-MIF Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-23
Question
Is a blocking peptide available for product anti-MIF Rabbit Monoclonal antibody (M00411)?
M. Wu
Verified customer
Asked: 2013-08-30
Answer
We do provide the blocking peptide for product anti-MIF Rabbit Monoclonal antibody (M00411). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-08-30
