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Product Info Summary
| SKU: | A03100-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Hsp40/DNAJB1 Antibody Picoband®
View all HSP40/DNAJB1 Antibodies
SKU/Catalog Number
A03100-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hsp40/DNAJB1 Antibody Picoband® catalog # A03100-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Hsp40/DNAJB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03100-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Hsp40/DNAJB1 recombinant protein (Position: M1-D313).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03100-2 is reactive to DNAJB1 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
64406 MW
Background of HSP40/DNAJB1
DnaJ homolog subfamily B member 1 is a protein that in humans is encoded by the DNAJB1 gene. This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03100-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human U251 whole cell, human PC-3 whole cell, human A549 whole cell, rat testis tissue
FCM: Daudi cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-Hsp40/DNAJB1 antibody (A03100-2).
Overlay histogram showing Daudi cells stained with A03100-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp40/DNAJB1 Antibody (A03100-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DNAJB1 (Source: Uniprot.org, NCBI)
Gene Name
DNAJB1
Full Name
DnaJ homolog subfamily B member 1
Weight
64406 MW
Alternative Names
NADPH oxidase 5; NOX5 DNAJB1 HSPF1, Hdj1, Hsp40, RSPH16B, Sis1 DnaJ heat shock protein family (Hsp40) member B1 dnaJ homolog subfamily B member 1|DnaJ (Hsp40) homolog, subfamily B, member 1|dnaJ protein homolog 1|heat shock 40 kDa protein 1|human DnaJ protein 1|radial spoke 16 homolog B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAJB1, check out the DNAJB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp40/DNAJB1 Antibody Picoband® (A03100-2)
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