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Product Info Summary
| SKU: | M03100 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9]
View all HSP40/DNAJB1 Antibodies
SKU/Catalog Number
M03100
Size
100 µl
Description
Boster Bio DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1E9 (formerly 1E9). Catalog# M03100. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03100)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1E9
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03100 is reactive to DNAJB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
37.9 kDa
Calculated molecular weight
38.044kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03100 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DNAJB1 using anti-DNAJB1 antibody (M03100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DNAJB1 antigen affinity purified monoclonal antibody (Catalog # M03100) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DNAJB1 at approximately 38 kDa. The expected band size for DNAJB1 is at 38 kDa.
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Flow cytometric Analysis of Hela cells
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Western blot analysis of extracts (10ug) from Hela cell line by using anti-DNAJB1 monoclonal antibody (1:200).
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HEK293T cells transfected with either DNAJB1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (M03100)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:100).
Protein Target Info & Infographic
Gene/Protein Information For DNAJB1 (Source: Uniprot.org, NCBI)
Gene Name
DNAJB1
Full Name
DnaJ homolog subfamily B member 1
Weight
38.044kDa
Alternative Names
DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1 DNAJB1 HSPF1, Hdj1, Hsp40, RSPH16B, Sis1 DnaJ heat shock protein family (Hsp40) member B1 dnaJ homolog subfamily B member 1|DnaJ (Hsp40) homolog, subfamily B, member 1|dnaJ protein homolog 1|heat shock 40 kDa protein 1|human DnaJ protein 1|radial spoke 16 homolog B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAJB1, check out the DNAJB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp40 (DNAJB1) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (M03100)
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