Anti-HSP27 Monoclonal Antibody
|Reactivity||Human, Mouse, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-HSP27 Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP27 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.|
|Reactivity||Human, Mouse, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Heat shock protein beta-1|
|Tissue Specificity||Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .|
|Alternative Names||Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;|
|Subcellular Localization||Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .|
|Molecular Weight||22893 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Involved in stress resistance and actin organization.|
|Research Areas||Human, Mouse, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]