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Product Info Summary
| SKU: | M05089 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5]
SKU/Catalog Number
M05089
Size
100 µl
Description
Boster Bio BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5). Catalog# M05089. Tested in IHC, WB. This antibody reacts with Human, Mouse, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05089)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3F5
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human BCAT1 (NP_005495) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05089 is reactive to BCAT1 in Dog, Human, Mouse
Reconstitution
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.966kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05089 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.
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Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Protein Target Info & Infographic
Gene/Protein Information For BCAT1 (Source: Uniprot.org, NCBI)
Gene Name
BCAT1
Full Name
Branched-chain-amino-acid aminotransferase, cytosolic
Weight
42.966kDa
Superfamily
class-IV pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
BCAT(c); BCAT1; BCAT-1; BCATC; BCT1; BCT1PNAS121; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; DKFZp686E12175; EC 2.6.1.42; ECA39; MECA39; placental protein 18; PNAS121; PP18; Protein ECA39 BCAT1 BCATC, BCT1, ECA39, MECA39, PNAS121, PP18 branched chain amino acid transaminase 1 branched-chain-amino-acid aminotransferase, cytosolic|branched chain amino-acid transaminase 1, cytosolic|branched chain aminotransferase 1, cytosolic|placental protein 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCAT1, check out the BCAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5] (M05089)
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