Anti-Hrk Antibody

Product Name

Anti-Hrk Antibody

View all HRK Antibodies

SKU/Catalog Number

A09125

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hrk Antibody (Catalog # A09125). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Hrk antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Hrk Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09125)

Host

Rabbit

Contents

Hrk Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members

Reactive Species

A09125 is reactive to HRK in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

9884 MW

Background of HRK

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09125 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For HRK (Source: Uniprot.org, NCBI)

Alternative Names

DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3 HRK DP5, HARAKIRI harakiri, BCL2 interacting protein activator of apoptosis harakiri|BH3-interacting domain-containing protein 3|activator of apoptosis Hrk|death protein 5|harakiri, BCL2 interacting protein (contains only BH3 domain)|neuronal death protein DP5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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