Anti-HOXA9 Antibody Picoband®

HOXA9 antibody

Boster Bio Anti-HOXA9 Antibody Picoband® catalog # PB9216. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9216
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-HOXA9 Antibody Picoband®

View all HOXA9 Antibodies

SKU/Catalog Number

PB9216

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HOXA9 Antibody Picoband® catalog # PB9216. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HOXA9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9216)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HOXA9 recombinant protein (Position: M1-R204). Human HOXA9 shares 98% amino acid (aa) sequence identity with mouse HOXA9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9216 is reactive to HOXA9 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

30 kDa

Calculated molecular weight

30172 MW

Background of HOXA9

Homeobox protein Hox-A9 is a protein that in humans is encoded by the HOXA9 gene. It is mapped to 7p15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA9 is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. As HOXA9 is part of the homeobox family, involved in setting the body plans of animals, it is likely that HOXA9 would display increased expression in cells with higher differentiation potentials.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9216 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Testis Tissue, HEPG2 Whole Cell, HEPA Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For HOXA9 (Source: Uniprot.org, NCBI)

Gene Name

HOXA9

Full Name

Homeobox protein Hox-A9

Weight

30172 MW

Superfamily

Abd-B homeobox family

Alternative Names

Homeobox protein Hox-A9;Homeobox protein Hox-1G;HOXA9;HOX1G; HOXA9 ABD-B, HOX1, HOX1.7, HOX1G homeobox A9 homeobox protein Hox-A9|homeobox protein Hox-1G|homeodomain protein HOXA9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HOXA9, check out the HOXA9 Infographic

HOXA9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HOXA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-HOXA9 Antibody Picoband®

Question

We have seen staining in rat colon. Are there any suggestions? Is anti-HOXA9 antibody supposed to stain colon positively?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

From literature colon does express HOXA9. From Uniprot.org, HOXA9 is expressed in metanephros, bone marrow, colon, among other tissues. Regarding which tissues have HOXA9 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9880515
Colon, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-08-19

Question

We are currently using anti-HOXA9 antibody PB9216 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The anti-HOXA9 antibody (PB9216) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-29

Question

Our lab were well pleased with the WB result of your anti-HOXA9 antibody. However we have observed positive staining in metanephros nucleus. using this antibody. Is that expected? Could you tell me where is HOXA9 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-05

Answer

Based on literature, metanephros does express HOXA9. Generally HOXA9 expresses in nucleus. Regarding which tissues have HOXA9 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9880515
Colon, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-04-05

Question

I was wanting to use using your anti-HOXA9 antibody for male gonad development studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.

R. Roberts

Verified customer

Asked: 2015-07-31

Answer

We appreciate your inquiry. This PB9216 anti-HOXA9 antibody is tested on rat testis tissue, tissue lysate, hepa whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-07-31

Order DetailsPrice
PB9216

100μg

$370
PB9216-10ug

10μg sample (liquid)

$99
PB9216-Biotin

100 μg Biotin conjugated

$570
PB9216-Cy3

100 μg Cy3 conjugated

$570
PB9216-Dylight488

100 μg Dylight488 conjugated

$570
PB9216-Dylight550

100 μg Dylight550 conjugated

$570
PB9216-Dylight594

100 μg Dylight594 conjugated

$570
PB9216-FITC

100 μg FITC conjugated

$570
PB9216-HRP

100 μg HRP conjugated

$570
PB9216-APC

100 μg APC conjugated

$670
PB9216-PE

100 μg PE conjugated

$670
PB9216-iFluor647

100 μg iFluor647 conjugated

$670
PB9216-carrier-free

Carrier Free

$370
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PB9216
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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